A second-generation phosphohistidine analog for production of phosphohistidine antibodies

Jung Min Kee; Rob C. Oslund; Anthony D. Couvillon; Tom W. Muir

doi:10.1021/ol503320p

A second-generation phosphohistidine analog for production of phosphohistidine antibodies

Jung Min Kee, Rob C. Oslund, Anthony D. Couvillon, Tom W. Muir

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Abstract

Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

Original language	English (US)
Pages (from-to)	187-189
Number of pages	3
Journal	Organic letters
Volume	17
Issue number	2
DOIs	https://doi.org/10.1021/ol503320p
State	Published - Jan 16 2015

All Science Journal Classification (ASJC) codes

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol503320p

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title = "A second-generation phosphohistidine analog for production of phosphohistidine antibodies",

abstract = "Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.",

author = "Kee, {Jung Min} and Oslund, {Rob C.} and Couvillon, {Anthony D.} and Muir, {Tom W.}",

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T1 - A second-generation phosphohistidine analog for production of phosphohistidine antibodies

AU - Kee, Jung Min

AU - Oslund, Rob C.

AU - Couvillon, Anthony D.

AU - Muir, Tom W.

PY - 2015/1/16

Y1 - 2015/1/16

N2 - Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

AB - Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

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JO - Organic letters

JF - Organic letters

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A second-generation phosphohistidine analog for production of phosphohistidine antibodies

Abstract

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