A second-generation phosphohistidine analog for production of phosphohistidine antibodies

Jung Min Kee, Rob C. Oslund, Anthony D. Couvillon, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

Original languageEnglish (US)
Pages (from-to)187-189
Number of pages3
JournalOrganic letters
Volume17
Issue number2
DOIs
StatePublished - Jan 16 2015

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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