TY - JOUR
T1 - A second domain of simian virus 40 T antigen in which mutations can alter the cellular localization of the antigen.
AU - Welsh, J. D.
AU - Swimmer, C.
AU - Cocke, T.
AU - Shenk, T.
PY - 1986/6
Y1 - 1986/6
N2 - Previous studies have demonstrated that mutations at amino acid position 128 of the simian virus 40 large T antigen can alter the subcellular localization of the antigen. A second domain in which mutations can alter localization of the nuclear antigen has been identified by mutations at amino acid positions 185, 186, and 199. Mutations in this region cause the polypeptide to accumulate in both the nucleus and cytoplasm of monkey cells. These T-antigen variants accumulate to near normal levels, but they don't bind to the simian virus 40 origin of DNA replication and are unable to mediate DNA replication. Furthermore, the altered tumor antigens can no longer transform secondary rat cells at normal efficiency, but they retain the ability to transform established mouse and rat cell lines.
AB - Previous studies have demonstrated that mutations at amino acid position 128 of the simian virus 40 large T antigen can alter the subcellular localization of the antigen. A second domain in which mutations can alter localization of the nuclear antigen has been identified by mutations at amino acid positions 185, 186, and 199. Mutations in this region cause the polypeptide to accumulate in both the nucleus and cytoplasm of monkey cells. These T-antigen variants accumulate to near normal levels, but they don't bind to the simian virus 40 origin of DNA replication and are unable to mediate DNA replication. Furthermore, the altered tumor antigens can no longer transform secondary rat cells at normal efficiency, but they retain the ability to transform established mouse and rat cell lines.
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U2 - 10.1128/MCB.6.6.2207
DO - 10.1128/MCB.6.6.2207
M3 - Article
C2 - 3023922
AN - SCOPUS:0022729856
SN - 0270-7306
VL - 6
SP - 2207
EP - 2212
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 6
ER -