A Review on Quantitative Multiplexed Proteomics

Nishant Pappireddi, Lance Martin, Martin Wühr

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

Over the last few decades, mass spectrometry-based proteomics has become an increasingly powerful tool that is now able to routinely detect and quantify thousands of proteins. A major advance for global protein quantification was the introduction of isobaric tags, which, in a single experiment, enabled the global quantification of proteins across multiple samples. Herein, these methods are referred to as multiplexed proteomics. The principles, advantages, and drawbacks of various multiplexed proteomics techniques are discussed and compared with alternative approaches. We also discuss how the emerging combination of multiplexing with targeted proteomics might enable the reliable and high-quality quantification of very low abundance proteins across multiple conditions. Lastly, we suggest that fusing multiplexed proteomics with data-independent acquisition approaches might enable the comparison of hundreds of different samples without missing values, while maintaining the superb measurement precision and accuracy obtainable with isobaric tag quantification.

Original languageEnglish (US)
Pages (from-to)1210-1224
Number of pages15
JournalChemBioChem
Volume20
Issue number10
DOIs
StatePublished - May 15 2019

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Keywords

  • analytical methods
  • isotopic labeling
  • mass spectrometry
  • proteins
  • proteomics

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