A quantitative rotational model for studying serotonergic function in the rat

Barry L. Jacobs, Sanford M. Simon, Daniel D. Ruimy, Michael E. Trulson

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Unilateral injection of 5,7-dihydroxytryptamine (4 μg/4 μl) into the medial forebrain bundle of rats produced serotonin depletions of 65% and 70% in the ipsilateral corpus striatum and ipsilateral forebrain, respectively. These animals showed a dose-dependent increase in contralateral turning (rotational behavior) when pretreated with a peripheral decarboxylase inhibitor and then injected with l-5-hydroxytryptophan in doses ranging from 5 to 100 mg/kg i.p. Injections of p-chloroamphetamine, which releases endogenous stores of serotonin, produced ipsilateral turning which could be blocked by prior serotonin depletion. Systemic administration of the catecholamine drugs l-DOPA, apomorphine and d-amphetamine never elicited consistent turning in either direction in these animals. These data indicate that the turning response of rats with unilateral destruction of brain serotonin nerve terminals provides a sensitive tool for quantifiably studying changes in serotonergic function.

Original languageEnglish (US)
Pages (from-to)271-281
Number of pages11
JournalBrain Research
Issue number2
StatePublished - Mar 25 1977

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Molecular Biology
  • General Neuroscience
  • Developmental Biology


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