A quantitative rotational model for studying serotonergic function in the rat

Barry L. Jacobs; Sanford M. Simon; Daniel D. Ruimy; Michael E. Trulson

doi:10.1016/0006-8993(77)90885-X

A quantitative rotational model for studying serotonergic function in the rat

Barry L. Jacobs, Sanford M. Simon, Daniel D. Ruimy, Michael E. Trulson

Psychology

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Unilateral injection of 5,7-dihydroxytryptamine (4 μg/4 μl) into the medial forebrain bundle of rats produced serotonin depletions of 65% and 70% in the ipsilateral corpus striatum and ipsilateral forebrain, respectively. These animals showed a dose-dependent increase in contralateral turning (rotational behavior) when pretreated with a peripheral decarboxylase inhibitor and then injected with l-5-hydroxytryptophan in doses ranging from 5 to 100 mg/kg i.p. Injections of p-chloroamphetamine, which releases endogenous stores of serotonin, produced ipsilateral turning which could be blocked by prior serotonin depletion. Systemic administration of the catecholamine drugs l-DOPA, apomorphine and d-amphetamine never elicited consistent turning in either direction in these animals. These data indicate that the turning response of rats with unilateral destruction of brain serotonin nerve terminals provides a sensitive tool for quantifiably studying changes in serotonergic function.

Original language	English (US)
Pages (from-to)	271-281
Number of pages	11
Journal	Brain Research
Volume	124
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(77)90885-X
State	Published - Mar 25 1977

All Science Journal Classification (ASJC) codes

Clinical Neurology
Molecular Biology
General Neuroscience
Developmental Biology

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10.1016/0006-8993(77)90885-X

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title = "A quantitative rotational model for studying serotonergic function in the rat",

abstract = "Unilateral injection of 5,7-dihydroxytryptamine (4 μg/4 μl) into the medial forebrain bundle of rats produced serotonin depletions of 65% and 70% in the ipsilateral corpus striatum and ipsilateral forebrain, respectively. These animals showed a dose-dependent increase in contralateral turning (rotational behavior) when pretreated with a peripheral decarboxylase inhibitor and then injected with l-5-hydroxytryptophan in doses ranging from 5 to 100 mg/kg i.p. Injections of p-chloroamphetamine, which releases endogenous stores of serotonin, produced ipsilateral turning which could be blocked by prior serotonin depletion. Systemic administration of the catecholamine drugs l-DOPA, apomorphine and d-amphetamine never elicited consistent turning in either direction in these animals. These data indicate that the turning response of rats with unilateral destruction of brain serotonin nerve terminals provides a sensitive tool for quantifiably studying changes in serotonergic function.",

author = "Jacobs, {Barry L.} and Simon, {Sanford M.} and Ruimy, {Daniel D.} and Trulson, {Michael E.}",

note = "Funding Information: This research was supported by Grants MH 23433 and MH 13445 from the National Institute of Mental Health, and the Spencer Foundation. We would like to thank Merck, Sharpe and Dohme (MK-486), Pfizer (PCPA) and Smith, Kline and French (D-amphetamine) for their generous gift of drugs.",

year = "1977",

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doi = "10.1016/0006-8993(77)90885-X",

language = "English (US)",

volume = "124",

pages = "271--281",

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T1 - A quantitative rotational model for studying serotonergic function in the rat

AU - Jacobs, Barry L.

AU - Simon, Sanford M.

AU - Ruimy, Daniel D.

AU - Trulson, Michael E.

N1 - Funding Information: This research was supported by Grants MH 23433 and MH 13445 from the National Institute of Mental Health, and the Spencer Foundation. We would like to thank Merck, Sharpe and Dohme (MK-486), Pfizer (PCPA) and Smith, Kline and French (D-amphetamine) for their generous gift of drugs.

PY - 1977/3/25

Y1 - 1977/3/25

N2 - Unilateral injection of 5,7-dihydroxytryptamine (4 μg/4 μl) into the medial forebrain bundle of rats produced serotonin depletions of 65% and 70% in the ipsilateral corpus striatum and ipsilateral forebrain, respectively. These animals showed a dose-dependent increase in contralateral turning (rotational behavior) when pretreated with a peripheral decarboxylase inhibitor and then injected with l-5-hydroxytryptophan in doses ranging from 5 to 100 mg/kg i.p. Injections of p-chloroamphetamine, which releases endogenous stores of serotonin, produced ipsilateral turning which could be blocked by prior serotonin depletion. Systemic administration of the catecholamine drugs l-DOPA, apomorphine and d-amphetamine never elicited consistent turning in either direction in these animals. These data indicate that the turning response of rats with unilateral destruction of brain serotonin nerve terminals provides a sensitive tool for quantifiably studying changes in serotonergic function.

AB - Unilateral injection of 5,7-dihydroxytryptamine (4 μg/4 μl) into the medial forebrain bundle of rats produced serotonin depletions of 65% and 70% in the ipsilateral corpus striatum and ipsilateral forebrain, respectively. These animals showed a dose-dependent increase in contralateral turning (rotational behavior) when pretreated with a peripheral decarboxylase inhibitor and then injected with l-5-hydroxytryptophan in doses ranging from 5 to 100 mg/kg i.p. Injections of p-chloroamphetamine, which releases endogenous stores of serotonin, produced ipsilateral turning which could be blocked by prior serotonin depletion. Systemic administration of the catecholamine drugs l-DOPA, apomorphine and d-amphetamine never elicited consistent turning in either direction in these animals. These data indicate that the turning response of rats with unilateral destruction of brain serotonin nerve terminals provides a sensitive tool for quantifiably studying changes in serotonergic function.

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A quantitative rotational model for studying serotonergic function in the rat

Abstract

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