@article{44b1d0731e294b05a16e24ff99dc72bd,
title = "A Quantitative Proteome Map of the Human Body",
abstract = "Determining protein levels in each tissue and how they compare with RNA levels is important for understanding human biology and disease as well as regulatory processes that control protein levels. We quantified the relative protein levels from over 12,000 genes across 32 normal human tissues. Tissue-specific or tissue-enriched proteins were identified and compared to transcriptome data. Many ubiquitous transcripts are found to encode tissue-specific proteins. Discordance of RNA and protein enrichment revealed potential sites of synthesis and action of secreted proteins. The tissue-specific distribution of proteins also provides an in-depth view of complex biological events that require the interplay of multiple tissues. Most importantly, our study demonstrated that protein tissue-enrichment information can explain phenotypes of genetic diseases, which cannot be obtained by transcript information alone. Overall, our results demonstrate how understanding protein levels can provide insights into regulation, secretome, metabolism, and human diseases.",
keywords = "BBS syndrome, Leigh syndrome, TMT, branched-chain amino acid metabolism, mass spectrometry, protein and RNA correlation, quantitative proteomics, secretion, tissue-enriched or -specific proteins",
author = "{GTEx Consortium} and Lihua Jiang and Meng Wang and Shin Lin and Ruiqi Jian and Xiao Li and Joanne Chan and Guanlan Dong and Huaying Fang and Robinson, {Aaron E.} and Fran{\c c}ois Aguet and Shankara Anand and Ardlie, {Kristin G.} and Stacey Gabriel and Gad Getz and Aaron Graubert and Kane Hadley and Handsaker, {Robert E.} and Huang, {Katherine H.} and Seva Kashin and MacArthur, {Daniel G.} and Meier, {Samuel R.} and Nedzel, {Jared L.} and Nguyen, {Duyen Y.} and Segr{\`e}, {Ayellet V.} and Ellen Todres and Brunilda Balliu and Barbeira, {Alvaro N.} and Alexis Battle and Rodrigo Bonazzola and Andrew Brown and Brown, {Christopher D.} and Castel, {Stephane E.} and Don Conrad and Cotter, {Daniel J.} and Nancy Cox and Sayantan Das and {de Goede}, {Olivia M.} and Dermitzakis, {Emmanouil T.} and Engelhardt, {Barbara E.} and Eleazar Eskin and Eulalio, {Tiffany Y.} and Ferraro, {Nicole M.} and Elise Flynn and Laure Fresard and Gamazon, {Eric R.} and Diego Garrido-Mart{\'i}n and Gay, {Nicole R.} and Roderic Guig{\'o} and Hamel, {Andrew R.} and Joshua Akey",
note = "Funding Information: We acknowledge the GTEx donors and families for donating organs to the GTEx Consortium. We thank K. Ardlie for coordinating the distribution of tissue samples. We also thank A. Breschi for discussion on RNA-seq data analysis and R. Tibshirani for suggestions on statistical analysis. We thank H. Tang for her help with experimental design and data analysis. Funding was provided by the NIH eGTEx grant ( 1U01HG007611-01 ) and NIH Center for Personal Dynamic Regulomes grant ( 3P50HG007735-05S1 ). Funding Information: We acknowledge the GTEx donors and families for donating organs to the GTEx Consortium. We thank K. Ardlie for coordinating the distribution of tissue samples. We also thank A. Breschi for discussion on RNA-seq data analysis and R. Tibshirani for suggestions on statistical analysis. We thank H. Tang for her help with experimental design and data analysis. Funding was provided by the NIH eGTEx grant (1U01HG007611-01) and NIH Center for Personal Dynamic Regulomes grant (3P50HG007735-05S1). L.J. led this project in generating proteomics data, data analysis, and manuscript preparation. M.W. developed statistical methods for proteomics data analysis and integration. S.L. performed the initial data analysis and SNP database construction. R.J. and J.C. did proteomics sample preparation and mass spectrometry data acquisition and contributed to making figures. X.L. did the analysis on the association of tissue-enriched proteins to diseases. H.F. contributed to the discussion of data analysis. G.D. contributed to SNP and isoform early data analysis. A.R. did SNP peptide spectra library search. M.P.S. contributed to project supervision and manuscript review and revision. All the authors contributed to manuscript revision. M.P.S. is a cofounder and is on the scientific advisory board of Personalis, Filtircine, SensOmics, Qbio, January, Mirvie, Oralome, and Proteus. He is also on the scientific advisory board (SAB) of Genapsys and Jupiter. The other authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = oct,
day = "1",
doi = "10.1016/j.cell.2020.08.036",
language = "English (US)",
volume = "183",
pages = "269--283.e19",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}