A polycomb and GAGA dependent silencer adjoins the Fab-7 boundary in the drosophila bithorax complex

Kirsten Hagstrom, Martin Muller, Paul Schedl

Research output: Contribution to journalArticle

161 Scopus citations

Abstract

The homeotic genes of the Drosophila bithorax complex are controlled by a large cis-regulatory region that ensures their segmentally restricted pattern of expression. A deletion that removes the Frontabdominal-7 cis- regulatory region (Fab-71) dominantly transforms parasegment 11 into parasegment 12. Previous studies suggested that removal of a domain boundary element on the proximal side of Fab71 is responsible for this gain-of- function phenotype. In this article we demonstrate that the Fab-71 deletion also removes a silencer element, the iab-7 PRE, which maps to a different DNA segment and plays a different role in regulating parasegment-specific expression patterns of the Abd-B gene. The lab7 PRE mediates pairing- sensitive silencing of mini-white, and can maintain the segmentally restricted expression pattern of a BXD, Ubx/lacZ reporter transgene. Both silencing activities depend upon Polycomb Group proteins. Pairing-sensitive silencing is relieved by removing the transvection protein Zeste, but is enhanced in a novel pairing-independent manner by the zeste1 allele. The iab-7PRE silencer is contained within a 0.8-kb fragment that spans a nuclease hypersensitive site, and silencing appears to depend on the chromatin remodeling protein, the GAGA factor.

Original languageEnglish (US)
Pages (from-to)1365-1380
Number of pages16
JournalGenetics
Volume146
Issue number4
StatePublished - Aug 22 1997

All Science Journal Classification (ASJC) codes

  • Genetics

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