A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.

J. Logan; E. Falck-Pedersen; J. E. Darnell; T. Shenk

doi:10.1073/pnas.84.23.8306

A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.

J. Logan, E. Falck-Pedersen, J. E. Darnell, T. Shenk

Molecular Biology

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191 Scopus citations

Abstract

Sequence elements within the mouse beta maj-globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chromosome within its E1A transcription unit. Two beta-globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of several single base pair substitutions into the AATAAA polyadenylylation motifs. These mutations inhibited both polyadenylylation and termination within the beta-globin DNA segment. Therefore, poly(A) addition appears to be a prerequisite for efficient termination.

Original language	English (US)
Pages (from-to)	8306-8310
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	23
DOIs	https://doi.org/10.1073/pnas.84.23.8306
State	Published - Dec 1987

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Logan, J., Falck-Pedersen, E., Darnell, J. E., & Shenk, T. (1987). A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene. Proceedings of the National Academy of Sciences of the United States of America, 84(23), 8306-8310. https://doi.org/10.1073/pnas.84.23.8306

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title = "A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.",

abstract = "Sequence elements within the mouse beta maj-globin transcription unit required for efficient termination of transcription by RNA polymerase II have been delineated. To facilitate nascent-chain analysis of termination, the DNA segment in which transcription ceases was introduced into the adenovirus chromosome within its E1A transcription unit. Two beta-globin DNA elements were required to effect efficient termination: an upstream sequence that includes two poly(A) addition signals and a downstream region previously shown to be where RNA synthesis stops. The role of poly(A) addition in termination was established by introduction of several single base pair substitutions into the AATAAA polyadenylylation motifs. These mutations inhibited both polyadenylylation and termination within the beta-globin DNA segment. Therefore, poly(A) addition appears to be a prerequisite for efficient termination.",

author = "J. Logan and E. Falck-Pedersen and Darnell, {J. E.} and T. Shenk",

year = "1987",

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Logan, J, Falck-Pedersen, E, Darnell, JE & Shenk, T 1987, 'A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.', Proceedings of the National Academy of Sciences of the United States of America, vol. 84, no. 23, pp. 8306-8310. https://doi.org/10.1073/pnas.84.23.8306

A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene. / Logan, J.; Falck-Pedersen, E.; Darnell, J. E. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 23, 12.1987, p. 8306-8310.

Research output: Contribution to journal › Article › peer-review

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A poly(A) addition site and a downstream termination region are required for efficient cessation of transcription by RNA polymerase II in the mouse beta maj-globin gene.

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