A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging

Yihua Zhao; Liwei Liu; Teng Luo; Liang Hong; Xiao Peng; Robert H. Austin; Junle Qu

doi:10.1016/j.snb.2018.04.154

A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging

Yihua Zhao, Liwei Liu, Teng Luo, Liang Hong, Xiao Peng, Robert H. Austin, Junle Qu

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We present a phosphorescence lifetime imaging (PLIM) based oxygen sensor to monitor the local O₂ level in conventional petri dish-based or microfluidic in vitro cell culture. The sensor film is fabricated by dissolving Pt(II) meso-tetrakis(pentafluorophenyl) porphine (PtTFPP) into a thermal curable poly(perfluoroether) (PFPE). The lifetime response of the sensor to O₂ follows a linear Stern-Volmer relationship. Compared with PDMS as polymer matrix, the use of PFPE as the polymer matrix for PtTFPP mitigates both the dye induced cytotoxicity and the biomolecule adsorption problems of polydimethylsiloxane (PDMS), while maintaining cell compatibility. By using the sensor film with PFPE as the polymer matrix, the O₂ tension during cell proliferation can be monitored in real time, and the resulting value can be used to calculate the per-cell O₂ consumption. We tested the application of the sensor for monitoring the O₂ tension during long term cell culture of HeLa cells and MDA-MB-231 cells, and calculated the O₂ consumption per cell for MDA-MB-231 cells. Our study demonstrates that the PtTFPP/PFPE sensor can be employed as a general noninvasive lifetime imaging platform for studying the di-oxygen in in vitro tissue models at the cellular level as a function of space and time.

Original language	English (US)
Pages (from-to)	88-95
Number of pages	8
Journal	Sensors and Actuators, B: Chemical
Volume	269
DOIs	https://doi.org/10.1016/j.snb.2018.04.154
State	Published - Sep 15 2018

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

Keywords

Cellular metabolism
Hypoxia
Oxygen sensor
Phosphorescence lifetime imaging (PLIM)

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title = "A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging",

abstract = "We present a phosphorescence lifetime imaging (PLIM) based oxygen sensor to monitor the local O2 level in conventional petri dish-based or microfluidic in vitro cell culture. The sensor film is fabricated by dissolving Pt(II) meso-tetrakis(pentafluorophenyl) porphine (PtTFPP) into a thermal curable poly(perfluoroether) (PFPE). The lifetime response of the sensor to O2 follows a linear Stern-Volmer relationship. Compared with PDMS as polymer matrix, the use of PFPE as the polymer matrix for PtTFPP mitigates both the dye induced cytotoxicity and the biomolecule adsorption problems of polydimethylsiloxane (PDMS), while maintaining cell compatibility. By using the sensor film with PFPE as the polymer matrix, the O2 tension during cell proliferation can be monitored in real time, and the resulting value can be used to calculate the per-cell O2 consumption. We tested the application of the sensor for monitoring the O2 tension during long term cell culture of HeLa cells and MDA-MB-231 cells, and calculated the O2 consumption per cell for MDA-MB-231 cells. Our study demonstrates that the PtTFPP/PFPE sensor can be employed as a general noninvasive lifetime imaging platform for studying the di-oxygen in in vitro tissue models at the cellular level as a function of space and time.",

keywords = "Cellular metabolism, Hypoxia, Oxygen sensor, Phosphorescence lifetime imaging (PLIM)",

author = "Yihua Zhao and Liwei Liu and Teng Luo and Liang Hong and Xiao Peng and Austin, {Robert H.} and Junle Qu",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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doi = "10.1016/j.snb.2018.04.154",

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T1 - A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging

AU - Zhao, Yihua

AU - Liu, Liwei

AU - Luo, Teng

AU - Hong, Liang

AU - Peng, Xiao

AU - Austin, Robert H.

AU - Qu, Junle

PY - 2018/9/15

Y1 - 2018/9/15

N2 - We present a phosphorescence lifetime imaging (PLIM) based oxygen sensor to monitor the local O2 level in conventional petri dish-based or microfluidic in vitro cell culture. The sensor film is fabricated by dissolving Pt(II) meso-tetrakis(pentafluorophenyl) porphine (PtTFPP) into a thermal curable poly(perfluoroether) (PFPE). The lifetime response of the sensor to O2 follows a linear Stern-Volmer relationship. Compared with PDMS as polymer matrix, the use of PFPE as the polymer matrix for PtTFPP mitigates both the dye induced cytotoxicity and the biomolecule adsorption problems of polydimethylsiloxane (PDMS), while maintaining cell compatibility. By using the sensor film with PFPE as the polymer matrix, the O2 tension during cell proliferation can be monitored in real time, and the resulting value can be used to calculate the per-cell O2 consumption. We tested the application of the sensor for monitoring the O2 tension during long term cell culture of HeLa cells and MDA-MB-231 cells, and calculated the O2 consumption per cell for MDA-MB-231 cells. Our study demonstrates that the PtTFPP/PFPE sensor can be employed as a general noninvasive lifetime imaging platform for studying the di-oxygen in in vitro tissue models at the cellular level as a function of space and time.

AB - We present a phosphorescence lifetime imaging (PLIM) based oxygen sensor to monitor the local O2 level in conventional petri dish-based or microfluidic in vitro cell culture. The sensor film is fabricated by dissolving Pt(II) meso-tetrakis(pentafluorophenyl) porphine (PtTFPP) into a thermal curable poly(perfluoroether) (PFPE). The lifetime response of the sensor to O2 follows a linear Stern-Volmer relationship. Compared with PDMS as polymer matrix, the use of PFPE as the polymer matrix for PtTFPP mitigates both the dye induced cytotoxicity and the biomolecule adsorption problems of polydimethylsiloxane (PDMS), while maintaining cell compatibility. By using the sensor film with PFPE as the polymer matrix, the O2 tension during cell proliferation can be monitored in real time, and the resulting value can be used to calculate the per-cell O2 consumption. We tested the application of the sensor for monitoring the O2 tension during long term cell culture of HeLa cells and MDA-MB-231 cells, and calculated the O2 consumption per cell for MDA-MB-231 cells. Our study demonstrates that the PtTFPP/PFPE sensor can be employed as a general noninvasive lifetime imaging platform for studying the di-oxygen in in vitro tissue models at the cellular level as a function of space and time.

KW - Cellular metabolism

KW - Hypoxia

KW - Oxygen sensor

KW - Phosphorescence lifetime imaging (PLIM)

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A platinum-porphine/poly(perfluoroether) film oxygen tension sensor for noninvasive local monitoring of cellular oxygen metabolism using phosphorescence lifetime imaging

Abstract

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