TY - JOUR
T1 - A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis
AU - Bartlett, Thomas M.
AU - Bratton, Benjamin P.
AU - Duvshani, Amit
AU - Martin, Nicholas R.
AU - Persat, Alexandre
AU - Gitai, Zemer
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/1/12
Y1 - 2017/1/12
N2 - Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm and thus can be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (quantitative analysis of sacculus architecture remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertions into the outer face than the inner face. Furthermore, crvA is quorum regulated, and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis.
AB - Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm and thus can be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (quantitative analysis of sacculus architecture remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertions into the outer face than the inner face. Furthermore, crvA is quorum regulated, and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis.
KW - bacterial cytoskeleton
KW - cell shape
KW - fluorescent D-amino acids
KW - pathogenesis
KW - peptidoglycan
KW - periskeleton
KW - quantitative cell biology
UR - http://www.scopus.com/inward/record.url?scp=85009160142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009160142&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2016.12.019
DO - 10.1016/j.cell.2016.12.019
M3 - Article
C2 - 28086090
AN - SCOPUS:85009160142
SN - 0092-8674
VL - 168
SP - 172-185.e15
JO - Cell
JF - Cell
IS - 1-2
ER -