A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis

Thomas M. Bartlett, Benjamin P. Bratton, Amit Duvshani, Nicholas R. Martin, Alexandre Persat, Zemer Gitai

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Pathogenic Vibrio cholerae remains a major human health concern. V. cholerae has a characteristic curved rod morphology, with a longer outer face and a shorter inner face. The mechanism and function of this curvature were previously unknown. Here, we identify and characterize CrvA, the first curvature determinant in V. cholerae. CrvA self-assembles into filaments at the inner face of cell curvature. Unlike traditional cytoskeletons, CrvA localizes to the periplasm and thus can be considered a periskeletal element. To quantify how curvature forms, we developed QuASAR (quantitative analysis of sacculus architecture remodeling), which measures subcellular peptidoglycan dynamics. QuASAR reveals that CrvA asymmetrically patterns peptidoglycan insertion rather than removal, causing more material insertions into the outer face than the inner face. Furthermore, crvA is quorum regulated, and CrvA-dependent curvature increases at high cell density. Finally, we demonstrate that CrvA promotes motility in hydrogels and confers an advantage in host colonization and pathogenesis.

Original languageEnglish (US)
Pages (from-to)172-185.e15
JournalCell
Volume168
Issue number1-2
DOIs
StatePublished - Jan 12 2017

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • bacterial cytoskeleton
  • cell shape
  • fluorescent D-amino acids
  • pathogenesis
  • peptidoglycan
  • periskeleton
  • quantitative cell biology

Fingerprint Dive into the research topics of 'A Periplasmic Polymer Curves Vibrio cholerae and Promotes Pathogenesis'. Together they form a unique fingerprint.

Cite this