A paternal–specific methylation imprint marks the alleles of the mouse H19 gene

Kimberly D. Tremblay, Jennifer R. Saam, Robert S. Ingram, Shirley M. Tilghman, Marisa S. Bartolomei

Research output: Contribution to journalArticlepeer-review

354 Scopus citations

Abstract

Imprinting, the differential expression of the two alleles of a gene based on their parental origin, requires that the alleles be distinguished or marked. A candidate for the differentiating mark is DNA methylation. The maternally expressed H19 gene is hypermethylated on the inactive paternal allele in somatic tissues and sperm, but to serve as the mark that designates the imprint, differential methylation must also be present in the gametes and the pre–implantation embryo. We now show that the pattern of differential methylation in the 5′ portion of H19 is established in the gametes and a subset is maintained in the pre–implanation embryo. That subset is sufficient to confer monoallelic expression to the gene in blastocysts. We propose that paternal–specific methylation of the far 5′ region is the mark that distinguishes the two alleles of H19.

Original languageEnglish (US)
Pages (from-to)407-413
Number of pages7
JournalNature Genetics
Volume9
Issue number4
DOIs
StatePublished - Apr 1995

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint

Dive into the research topics of 'A paternal–specific methylation imprint marks the alleles of the mouse H19 gene'. Together they form a unique fingerprint.

Cite this