A novel function for dendritic cell: Clearance of VEGF via VEGF receptor-1

Yi Xie, Jianqing Fan, Juhua Chen, Fang Ping Huang, Brian Cao, Paul K.H. Tam, Yi Ren

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

It has been reported that the plasma levels of VEGF in tumor patients decreased during dendritic cell (DC)-based immunotherapy, but the underlying mechanism remains unclear. Our current report demonstrates that VEGF levels were significantly decreased in the supernatants of DCs incubated with rhVEGF or tumor conditioned medium (TCM) while the intracellular VEGF in DCs was increased. The increased intracellular VEGF was not due to the de novo VEGF synthesis by DCs because exogenous VEGF inhibited the mRNA expression of VEGF in DCs. More direct evidence was provided to demonstrate that Cy3-labeled VEGF could be internalized by DCs specifically and efficiently. In addition, the activity of DCs to internalize VEGF was abolished by neutralizing antibody against VEGF receptor-1 (Flt-1) and inhibitors of endocytosis such as carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and genistein. This study highlights a novel function of DCs and allows a better understanding of the DC-VEGF interaction.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number2
DOIs
StatePublished - Mar 6 2009

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • DC
  • VEGF
  • VEGF receptor-1

Fingerprint Dive into the research topics of 'A novel function for dendritic cell: Clearance of VEGF via VEGF receptor-1'. Together they form a unique fingerprint.

Cite this