TY - JOUR
T1 - A novel cis-regulatory element, the PTS, mediates an anti-insulator activity in the Drosophila embryo
AU - Zhou, Jumin
AU - Levine, Michael
N1 - Funding Information:
We thank Connie Liang, Jennifer Gin, and Brian Castro for technical assistance; Ian Duncan, Shigeru Sakonju, Steve Small, and Gary Struhl for kindly providing fly strains; and Hilary Ashe, Dale Dorsett, Dave Keys, Jürg Müller, Marcus Noll, and Angela Stathopoulos for critically reading the manuscript. This work was funded by a grant from the National Institutes of Health (GM 34431). J. Z. is a fellow of the National Institutes of Health (GM 19329).
PY - 1999/12/10
Y1 - 1999/12/10
N2 - The Abd-B Hox gene contains an extended 3' cis-regulatory region that is subdivided into a series of separate lab domains. The iab-7 domain activates Abd-B in parasegment 12 (ps12), whereas iab-8 controls expression in ps13. iab-7 is flanked by two insulators, Fab-7 and Fab-8, which are thought to prevent regulatory factors, such as Polycomb silencers, from influencing neighboring iab domains. This organization poses a potential paradox, since insulator DNAs can work in a dominant fashion to block enhancer-promoter interactions over long distances. Here, we present evidence for a novel cis- regulatory sequence located within iab-7, the promoter targeting sequence (PTS), which permits distal enhancers to overcome the blocking effects of Fab-8 and the heterologous su(Hw) insulator. We propose that the PTS converts dominant, long-range insulators into local regulatory elements that separate neighboring iab domains.
AB - The Abd-B Hox gene contains an extended 3' cis-regulatory region that is subdivided into a series of separate lab domains. The iab-7 domain activates Abd-B in parasegment 12 (ps12), whereas iab-8 controls expression in ps13. iab-7 is flanked by two insulators, Fab-7 and Fab-8, which are thought to prevent regulatory factors, such as Polycomb silencers, from influencing neighboring iab domains. This organization poses a potential paradox, since insulator DNAs can work in a dominant fashion to block enhancer-promoter interactions over long distances. Here, we present evidence for a novel cis- regulatory sequence located within iab-7, the promoter targeting sequence (PTS), which permits distal enhancers to overcome the blocking effects of Fab-8 and the heterologous su(Hw) insulator. We propose that the PTS converts dominant, long-range insulators into local regulatory elements that separate neighboring iab domains.
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U2 - 10.1016/S0092-8674(00)81546-9
DO - 10.1016/S0092-8674(00)81546-9
M3 - Article
C2 - 10612393
AN - SCOPUS:0033544703
SN - 0092-8674
VL - 99
SP - 567
EP - 575
JO - Cell
JF - Cell
IS - 6
ER -