A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome

Bayar Thimmappaya; Nicholas Jones; Thomas Shenk

doi:10.1016/0092-8674(79)90207-1

A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome

Bayar Thimmappaya, Nicholas Jones, Thomas Shenk

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40 Scopus citations

Abstract

Mutant dl 309 is a viable Ad5 deletion mutant. Whereas wild-type Ad5-infected HeLa cells contain two VAI RNA species [VAI(A) and VAI(G)] which differ by three nucleotides at their 5′ ends, dl 309-infected HeLa cells contain VAI(G) but no VAI(A) RNA. Nucleotide sequence analysis indicates that dl 309 lacks two base pairs which precede the 5′ end of VAI(A) by 22 nucleotides. Since the 5′ ends of VAI RNAs are not processed, the 309 deletion serves to identify a portion of the sequence required for RNA polymerase III initiation. Since dl 309 grows as well as wild-type Ad5 in HeLa cells, the VAI(A) species is not essential for viral growth in these cells.

Original language	English (US)
Pages (from-to)	947-954
Number of pages	8
Journal	Cell
Volume	18
Issue number	4
DOIs	https://doi.org/10.1016/0092-8674(79)90207-1
State	Published - Dec 1979

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/0092-8674(79)90207-1

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title = "A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome",

abstract = "Mutant dl 309 is a viable Ad5 deletion mutant. Whereas wild-type Ad5-infected HeLa cells contain two VAI RNA species [VAI(A) and VAI(G)] which differ by three nucleotides at their 5′ ends, dl 309-infected HeLa cells contain VAI(G) but no VAI(A) RNA. Nucleotide sequence analysis indicates that dl 309 lacks two base pairs which precede the 5′ end of VAI(A) by 22 nucleotides. Since the 5′ ends of VAI RNAs are not processed, the 309 deletion serves to identify a portion of the sequence required for RNA polymerase III initiation. Since dl 309 grows as well as wild-type Ad5 in HeLa cells, the VAI(A) species is not essential for viral growth in these cells.",

author = "Bayar Thimmappaya and Nicholas Jones and Thomas Shenk",

note = "Funding Information: We acknowledge the competent technical assistance of Ms. Bonnie Swerdlow. We thank Dr. Sherman Weissman for allowing us to perform the two-dimensional oligonucleotide fractionations in his laboratory, This work was supported by a grant from the American Cancer Society. T.S. is an Established Investigator of the American Heart Association.",

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journal = "Cell",

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T1 - A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome

AU - Thimmappaya, Bayar

AU - Jones, Nicholas

AU - Shenk, Thomas

N1 - Funding Information: We acknowledge the competent technical assistance of Ms. Bonnie Swerdlow. We thank Dr. Sherman Weissman for allowing us to perform the two-dimensional oligonucleotide fractionations in his laboratory, This work was supported by a grant from the American Cancer Society. T.S. is an Established Investigator of the American Heart Association.

PY - 1979/12

Y1 - 1979/12

N2 - Mutant dl 309 is a viable Ad5 deletion mutant. Whereas wild-type Ad5-infected HeLa cells contain two VAI RNA species [VAI(A) and VAI(G)] which differ by three nucleotides at their 5′ ends, dl 309-infected HeLa cells contain VAI(G) but no VAI(A) RNA. Nucleotide sequence analysis indicates that dl 309 lacks two base pairs which precede the 5′ end of VAI(A) by 22 nucleotides. Since the 5′ ends of VAI RNAs are not processed, the 309 deletion serves to identify a portion of the sequence required for RNA polymerase III initiation. Since dl 309 grows as well as wild-type Ad5 in HeLa cells, the VAI(A) species is not essential for viral growth in these cells.

AB - Mutant dl 309 is a viable Ad5 deletion mutant. Whereas wild-type Ad5-infected HeLa cells contain two VAI RNA species [VAI(A) and VAI(G)] which differ by three nucleotides at their 5′ ends, dl 309-infected HeLa cells contain VAI(G) but no VAI(A) RNA. Nucleotide sequence analysis indicates that dl 309 lacks two base pairs which precede the 5′ end of VAI(A) by 22 nucleotides. Since the 5′ ends of VAI RNAs are not processed, the 309 deletion serves to identify a portion of the sequence required for RNA polymerase III initiation. Since dl 309 grows as well as wild-type Ad5 in HeLa cells, the VAI(A) species is not essential for viral growth in these cells.

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A mutation which alters initiation of transcription by RNA polymerase III on the Ad5 chromosome

Abstract

All Science Journal Classification (ASJC) codes

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