TY - JOUR
T1 - A multiplex fluorescent in situ hybridization protocol for clonal analysis of drosophila oogenesis
AU - Cheung, Lily S.
AU - Shvartsman, Stanislav
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2015.
PY - 2015
Y1 - 2015
N2 - Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.
AB - Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.
KW - Drosophila melanogaster
KW - Fluorescent in situ hybridization (FISH)
KW - Follicle cells
KW - Mosaic analysis with a repressible cell marker (MARCM)
KW - Oogenesis
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U2 - 10.1007/978-1-4939-1164-6_8
DO - 10.1007/978-1-4939-1164-6_8
M3 - Article
C2 - 25245690
AN - SCOPUS:84954620095
SN - 1064-3745
VL - 1189
SP - 115
EP - 122
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -