A multiplex fluorescent in situ hybridization protocol for clonal analysis of drosophila oogenesis

Lily S. Cheung, Stanislav Shvartsman

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.

Original languageEnglish (US)
Pages (from-to)115-122
Number of pages8
JournalMethods in Molecular Biology
Volume1189
DOIs
StatePublished - Jan 1 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Drosophila melanogaster
  • Fluorescent in situ hybridization (FISH)
  • Follicle cells
  • Mosaic analysis with a repressible cell marker (MARCM)
  • Oogenesis

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