A mouse model for HIV-1 entry

John Pietzsch; Henning Gruell; Stylianos Bournazos; Bridget M. Donovan; Florian Klein; Ron Diskin; Michael S. Seaman; Pamela J. Bjorkman; Jeffrey V. Ravetch; Alexander Ploss; Michel C. Nussenzweig

doi:10.1073/pnas.1213409109

A mouse model for HIV-1 entry

John Pietzsch, Henning Gruell, Stylianos Bournazos, Bridget M. Donovan, Florian Klein, Ron Diskin, Michael S. Seaman, Pamela J. Bjorkman, Jeffrey V. Ravetch, Alexander Ploss, Michel C. Nussenzweig

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64 Scopus citations

Abstract

Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.

Original language	English (US)
Pages (from-to)	15859-15864
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	109
Issue number	39
DOIs	https://doi.org/10.1073/pnas.1213409109
State	Published - Sep 25 2012

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AU - Pietzsch, John

AU - Gruell, Henning

AU - Bournazos, Stylianos

AU - Donovan, Bridget M.

AU - Klein, Florian

AU - Diskin, Ron

AU - Seaman, Michael S.

AU - Bjorkman, Pamela J.

AU - Ravetch, Jeffrey V.

AU - Ploss, Alexander

AU - Nussenzweig, Michel C.

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AB - Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.

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A mouse model for HIV-1 entry

Abstract

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