TY - JOUR
T1 - A mouse model for HIV-1 entry
AU - Pietzsch, John
AU - Gruell, Henning
AU - Bournazos, Stylianos
AU - Donovan, Bridget M.
AU - Klein, Florian
AU - Diskin, Ron
AU - Seaman, Michael S.
AU - Bjorkman, Pamela J.
AU - Ravetch, Jeffrey V.
AU - Ploss, Alexander
AU - Nussenzweig, Michel C.
PY - 2012/9/25
Y1 - 2012/9/25
N2 - Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.
AB - Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.
UR - http://www.scopus.com/inward/record.url?scp=84866843530&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866843530&partnerID=8YFLogxK
U2 - 10.1073/pnas.1213409109
DO - 10.1073/pnas.1213409109
M3 - Article
C2 - 23019371
AN - SCOPUS:84866843530
SN - 0027-8424
VL - 109
SP - 15859
EP - 15864
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 39
ER -