A monomer-trimer model supports intermittent glucagon fibril growth

Andrej Košmrlj, Pia Cordsen, Anders Kyrsting, Daniel E. Otzen, Lene B. Oddershede, Mogens H. Jensen

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We investigate in vitro fibrillation kinetics of the hormone peptide glucagon at various concentrations using confocal microscopy and determine the glucagon fibril persistence length 60μm. At all concentrations we observe that periods of individual fibril growth are interrupted by periods of stasis. The growth probability is large at high and low concentrations and is reduced for intermediate glucagon concentrations. To explain this behavior we propose a simple model, where fibrils come in two forms, one built entirely from glucagon monomers and one entirely from glucagon trimers. The opposite building blocks act as fibril growth blockers, and this generic model reproduces experimental behavior well.

Original languageEnglish (US)
Article number9005
JournalScientific reports
Volume5
DOIs
StatePublished - Mar 11 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

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    Košmrlj, A., Cordsen, P., Kyrsting, A., Otzen, D. E., Oddershede, L. B., & Jensen, M. H. (2015). A monomer-trimer model supports intermittent glucagon fibril growth. Scientific reports, 5, [9005]. https://doi.org/10.1038/srep09005