A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz; Katsuhiko Kamada; Stephen K. Burley; David Fenyö; Brian T. Chait; Tom W. Muir

A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz
, Katsuhiko Kamada
, Stephen K. Burley
, David Fenyö
, Brian T. Chait
, Tom W. Muir

Research output: Contribution to conference › Paper › peer-review

Abstract

The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.

Original language	English (US)
Pages	273-274
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

All Science Journal Classification (ASJC) codes

Spectroscopy

Cite this

@conference{0591db1088e4443daaacae02031bf5d4,

title = "A modular cross-linking approach for exploring protein interactions",

abstract = "The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.",

author = "Michelle Trester-Zedlitz and Katsuhiko Kamada and Burley, \{Stephen K.\} and David Feny{\"o} and Chait, \{Brian T.\} and Muir, \{Tom W.\}",

year = "2002",

language = "English (US)",

pages = "273--274",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - A modular cross-linking approach for exploring protein interactions

AU - Trester-Zedlitz, Michelle

AU - Kamada, Katsuhiko

AU - Burley, Stephen K.

AU - Fenyö, David

AU - Chait, Brian T.

AU - Muir, Tom W.

PY - 2002

Y1 - 2002

N2 - The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.

AB - The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.

UR - https://www.scopus.com/pages/publications/2442689387

UR - https://www.scopus.com/pages/publications/2442689387#tab=citedBy

M3 - Paper

AN - SCOPUS:2442689387

SP - 273

EP - 274

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

A modular cross-linking approach for exploring protein interactions

Abstract

Other

All Science Journal Classification (ASJC) codes

Other files and links

Fingerprint

Cite this