A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz; Katsuhiko Kamada; Stephen K. Burley; David Fenyö; Brian T. Chait; Tom W. Muir

doi:10.1021/ja026917a

A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen K. Burley, David Fenyö, Brian T. Chait, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

168 Scopus citations

Abstract

A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

Original language	English (US)
Pages (from-to)	2416-2425
Number of pages	10
Journal	Journal of the American Chemical Society
Volume	125
Issue number	9
DOIs	https://doi.org/10.1021/ja026917a
State	Published - Mar 5 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

Access to Document

10.1021/ja026917a

Cite this

@article{2121856185144234a6ccc665fc03392e,

title = "A modular cross-linking approach for exploring protein interactions",

abstract = "A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.",

author = "Michelle Trester-Zedlitz and Katsuhiko Kamada and Burley, \{Stephen K.\} and David Feny{\"o} and Chait, \{Brian T.\} and Muir, \{Tom W.\}",

year = "2003",

month = mar,

day = "5",

doi = "10.1021/ja026917a",

language = "English (US)",

volume = "125",

pages = "2416--2425",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "9",

}

TY - JOUR

T1 - A modular cross-linking approach for exploring protein interactions

AU - Trester-Zedlitz, Michelle

AU - Kamada, Katsuhiko

AU - Burley, Stephen K.

AU - Fenyö, David

AU - Chait, Brian T.

AU - Muir, Tom W.

PY - 2003/3/5

Y1 - 2003/3/5

N2 - A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

AB - A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

UR - https://www.scopus.com/pages/publications/0242669340

UR - https://www.scopus.com/inward/citedby.url?scp=0242669340&partnerID=8YFLogxK

U2 - 10.1021/ja026917a

DO - 10.1021/ja026917a

M3 - Article

C2 - 12603129

AN - SCOPUS:0242669340

SN - 0002-7863

VL - 125

SP - 2416

EP - 2425

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 9

ER -

A modular cross-linking approach for exploring protein interactions

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this