A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen K. Burley, David Fenyö, Brian T. Chait, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

165 Scopus citations


A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

Original languageEnglish (US)
Pages (from-to)2416-2425
Number of pages10
JournalJournal of the American Chemical Society
Issue number9
StatePublished - Mar 5 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Biochemistry
  • Catalysis
  • Colloid and Surface Chemistry


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