A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz; Katsuhiko Kamada; Stephen K. Burley; David Fenyö; Brian T. Chait; Tom W. Muir

doi:10.1021/ja026917a

A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen K. Burley, David Fenyö, Brian T. Chait, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

166 Scopus citations

Abstract

A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

Original language	English (US)
Pages (from-to)	2416-2425
Number of pages	10
Journal	Journal of the American Chemical Society
Volume	125
Issue number	9
DOIs	https://doi.org/10.1021/ja026917a
State	Published - Mar 5 2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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10.1021/ja026917a

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AU - Trester-Zedlitz, Michelle

AU - Kamada, Katsuhiko

AU - Burley, Stephen K.

AU - Fenyö, David

AU - Chait, Brian T.

AU - Muir, Tom W.

PY - 2003/3/5

Y1 - 2003/3/5

N2 - A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

AB - A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

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JF - Journal of the American Chemical Society

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A modular cross-linking approach for exploring protein interactions

Abstract

All Science Journal Classification (ASJC) codes

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