Abstract
The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.
Original language | English (US) |
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Pages | 273-274 |
Number of pages | 2 |
State | Published - 2002 |
Externally published | Yes |
Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
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Country/Territory | United States |
City | Orlando, FL |
Period | 6/2/02 → 6/6/02 |
All Science Journal Classification (ASJC) codes
- Spectroscopy