A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz, Katsuhiko Kamada, Stephen K. Burley, David Fenyö, Brian T. Chait, Tom W. Muir

Research output: Contribution to conferencePaperpeer-review

Abstract

The protein-protein interaction was described using cross-linking reagents and mass spectrometry. This approach was applied for the study of cross-linking between the components of the heterodimeric protein complex, Negative Cofactor 2 (NC2). Five crosslinking reagents were synthesized using solid phase chemistry, by availing the chemical versatility of the mercaptopropionamide-PEGA resin-linker. It was found that there is an intermolecular interaction between the N-terminal regions of NC2 α and β, as well as three intra-molecular and several dead-end cross-links.

Original languageEnglish (US)
Pages273-274
Number of pages2
StatePublished - Dec 1 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Fingerprint Dive into the research topics of 'A modular cross-linking approach for exploring protein interactions'. Together they form a unique fingerprint.

Cite this