A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces

Liyu Liu, Kevin Loutherback, David Liao, David Yeater, Guillaume Lambert, André Estévez-Torres, James C. Sturm, Robert H. Getzenberg, Robert H. Austin

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

We demonstrate a novel and robust microfluidic chip with combined functions of continuous culture and output of PC-3 prostate cancer cells. With digital controls, polydimethylsiloxane (PDMS) flexible diaphragms are able to apply hydrodynamic shear forces on cultures, detaching a fraction of attached cancer cells from the surface for output while leaving others for reuse in subsequent cultures. The fractions of detached cells and remaining cells can be precisely controlled. The system has not only the advantages of small size, high cell culture efficiency, and digital control, but also of simple fabrication at low cost, easy operation and robust performance. The chip performs 9 passages during 30 days of continuous culture and shows promise as a durable design suitable for long-term cell output.

Original languageEnglish (US)
Pages (from-to)1807-1813
Number of pages7
JournalLab on a Chip
Volume10
Issue number14
DOIs
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces'. Together they form a unique fingerprint.

  • Cite this

    Liu, L., Loutherback, K., Liao, D., Yeater, D., Lambert, G., Estévez-Torres, A., Sturm, J. C., Getzenberg, R. H., & Austin, R. H. (2010). A microfluidic device for continuous cancer cell culture and passage with hydrodynamic forces. Lab on a Chip, 10(14), 1807-1813. https://doi.org/10.1039/c003509b