A method for the measurement of choline and hydrogen peroxide in seawater

M. A. Roulier; B. Palenik; Francois M. M. Morel

doi:10.1016/0304-4203(90)90084-P

A method for the measurement of choline and hydrogen peroxide in seawater

M. A. Roulier, B. Palenik, Francois M. M. Morel

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H₂O₂, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.

Original language	English (US)
Pages (from-to)	409-421
Number of pages	13
Journal	Marine Chemistry
Volume	30
Issue number	C
DOIs	https://doi.org/10.1016/0304-4203(90)90084-P
State	Published - 1990

All Science Journal Classification (ASJC) codes

Oceanography
General Chemistry
Environmental Chemistry
Water Science and Technology

Access to Document

10.1016/0304-4203(90)90084-P

Cite this

@article{548ee5bcb0e548a4bb7176dd94bacaef,

title = "A method for the measurement of choline and hydrogen peroxide in seawater",

abstract = "The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H2O2, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.",

author = "Roulier, {M. A.} and B. Palenik and Morel, {Francois M. M.}",

note = "Funding Information: This work was supportebdy NSF grant8 615545-OCEO, NR grantN 00014-86-K-0325a, nda lsob y MIT Sea Grant and UROP programs.",

year = "1990",

doi = "10.1016/0304-4203(90)90084-P",

language = "English (US)",

volume = "30",

pages = "409--421",

journal = "Marine Chemistry",

issn = "0304-4203",

publisher = "Elsevier B.V.",

number = "C",

}

TY - JOUR

T1 - A method for the measurement of choline and hydrogen peroxide in seawater

AU - Roulier, M. A.

AU - Palenik, B.

AU - Morel, Francois M. M.

N1 - Funding Information: This work was supportebdy NSF grant8 615545-OCEO, NR grantN 00014-86-K-0325a, nda lsob y MIT Sea Grant and UROP programs.

PY - 1990

Y1 - 1990

N2 - The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H2O2, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.

AB - The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H2O2, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.

UR - http://www.scopus.com/inward/record.url?scp=0012555073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0012555073&partnerID=8YFLogxK

U2 - 10.1016/0304-4203(90)90084-P

DO - 10.1016/0304-4203(90)90084-P

M3 - Article

AN - SCOPUS:0012555073

SN - 0304-4203

VL - 30

SP - 409

EP - 421

JO - Marine Chemistry

JF - Marine Chemistry

IS - C

ER -

A method for the measurement of choline and hydrogen peroxide in seawater

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this