TY - JOUR
T1 - A method for the measurement of choline and hydrogen peroxide in seawater
AU - Roulier, M. A.
AU - Palenik, B.
AU - Morel, Francois M. M.
N1 - Funding Information:
This work was supportebdy NSF grant8 615545-OCEO, NR grantN 00014-86-K-0325a, nda lsob y MIT Sea Grant and UROP programs.
PY - 1990
Y1 - 1990
N2 - The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H2O2, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.
AB - The horseradish peroxidase-mediated dimerization of hydroxyphenylpropionic acid can be used to measure hydrogen peroxide in seawater. The method was optimized and interferences investigated, and the method was then adapted to the measurement of choline in seawater. The enzyme choline oxidase is used specifically to oxidize choline to produce betaine and H2O2, and the latter is measured. Possible interferences with this method were investigated, and choline was measured in coastal seawater, where it varied from 0 to 45 nM.
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U2 - 10.1016/0304-4203(90)90084-P
DO - 10.1016/0304-4203(90)90084-P
M3 - Article
AN - SCOPUS:0012555073
SN - 0304-4203
VL - 30
SP - 409
EP - 421
JO - Marine Chemistry
JF - Marine Chemistry
IS - C
ER -