A method for single-neuron chronic recording from the retina in awake mice

Guosong Hong; Tian Ming Fu; Mu Qiao; Robert D. Viveros; Xiao Yang; Tao Zhou; Jung Min Lee; Hong Gyu Park; Joshua R. Sanes; Charles M. Lieber

doi:10.1126/science.aas9160

A method for single-neuron chronic recording from the retina in awake mice

Guosong Hong, Tian Ming Fu, Mu Qiao, Robert D. Viveros, Xiao Yang, Tao Zhou, Jung Min Lee, Hong Gyu Park, Joshua R. Sanes, Charles M. Lieber

Research output: Contribution to journal › Article › peer-review

128 Scopus citations

Abstract

The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

Original language	English (US)
Pages (from-to)	1447-1451
Number of pages	5
Journal	Science
Volume	360
Issue number	6396
DOIs	https://doi.org/10.1126/science.aas9160
State	Published - Jun 29 2018
Externally published	Yes

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abstract = "The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.",

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AU - Hong, Guosong

AU - Fu, Tian Ming

AU - Qiao, Mu

AU - Viveros, Robert D.

AU - Yang, Xiao

AU - Zhou, Tao

AU - Lee, Jung Min

AU - Park, Hong Gyu

AU - Sanes, Joshua R.

AU - Lieber, Charles M.

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AB - The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

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A method for single-neuron chronic recording from the retina in awake mice

Abstract

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