A method for single-neuron chronic recording from the retina in awake mice

Guosong Hong, Tian Ming Fu, Mu Qiao, Robert D. Viveros, Xiao Yang, Tao Zhou, Jung Min Lee, Hong Gyu Park, Joshua R. Sanes, Charles M. Lieber

Research output: Contribution to journalArticlepeer-review

121 Scopus citations


The retina, which processes visual information and sends it to the brain, is an excellent model for studying neural circuitry. It has been probed extensively ex vivo but has been refractory to chronic in vivo electrophysiology. We report a nonsurgical method to achieve chronically stable in vivo recordings from single retinal ganglion cells (RGCs) in awake mice. We developed a noncoaxial intravitreal injection scheme in which injected mesh electronics unrolls inside the eye and conformally coats the highly curved retina without compromising normal eye functions. The method allows 16-channel recordings from multiple types of RGCs with stable responses to visual stimuli for at least 2 weeks, and reveals circadian rhythms in RGC responses over multiple day/night cycles.

Original languageEnglish (US)
Pages (from-to)1447-1451
Number of pages5
Issue number6396
StatePublished - Jun 29 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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