A marmoset brain cell census reveals regional specialization of cellular identities

Fenna M. Krienen, Kirsten M. Levandowski, Heather Zaniewski, Ricardo C.H. del Rosario, Margaret E. Schroeder, Melissa Goldman, Martin Wienisch, Alyssa Lutservitz, Victoria F. Beja-Glasser, Cindy Chen, Qiangge Zhang, Ken Y. Chan, Katelyn X. Li, Jitendra Sharma, Dana McCormack, Tay Won Shin, Andrew Harrahill, Eric Nyase, Gagandeep Mudhar, Abigail MauermannAlec Wysoker, James Nemesh, Seva Kashin, Josselyn Vergara, Gabriele Chelini, Jordane Dimidschstein, Sabina Berretta, Benjamin E. Deverman, Ed Boyden, Steven A. McCarroll, Guoping Feng

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The mammalian brain is composed of many brain structures, each with its own ontogenetic and developmental history. We used single-nucleus RNA sequencing to sample over 2.4 million brain cells across 18 locations in the common marmoset, a New World monkey primed for genetic engineering, and examined gene expression patterns of cell types within and across brain structures. The adult transcriptomic identity of most neuronal types is shaped more by developmental origin than by neurotransmitter signaling repertoire. Quantitative mapping of GABAergic types with single-molecule FISH (smFISH) reveals that interneurons in the striatum and neocortex follow distinct spatial principles, and that lateral prefrontal and other higher-order cortical association areas are distinguished by high proportions of VIP+ neurons. We use cell type–specific enhancers to drive AAV-GFP and reconstruct the morphologies of molecularly resolved interneuron types in neocortex and striatum. Our analyses highlight how lineage, local context, and functional class contribute to the transcriptional identity and biodistribution of primate brain cell types.

Original languageEnglish (US)
Article numbereadk3986
JournalScience Advances
Volume9
Issue number41
DOIs
StatePublished - 2023
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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