TY - JOUR
T1 - A marmoset brain cell census reveals regional specialization of cellular identities
AU - Krienen, Fenna M.
AU - Levandowski, Kirsten M.
AU - Zaniewski, Heather
AU - del Rosario, Ricardo C.H.
AU - Schroeder, Margaret E.
AU - Goldman, Melissa
AU - Wienisch, Martin
AU - Lutservitz, Alyssa
AU - Beja-Glasser, Victoria F.
AU - Chen, Cindy
AU - Zhang, Qiangge
AU - Chan, Ken Y.
AU - Li, Katelyn X.
AU - Sharma, Jitendra
AU - McCormack, Dana
AU - Shin, Tay Won
AU - Harrahill, Andrew
AU - Nyase, Eric
AU - Mudhar, Gagandeep
AU - Mauermann, Abigail
AU - Wysoker, Alec
AU - Nemesh, James
AU - Kashin, Seva
AU - Vergara, Josselyn
AU - Chelini, Gabriele
AU - Dimidschstein, Jordane
AU - Berretta, Sabina
AU - Deverman, Benjamin E.
AU - Boyden, Ed
AU - McCarroll, Steven A.
AU - Feng, Guoping
N1 - Publisher Copyright:
© 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).
PY - 2023
Y1 - 2023
N2 - The mammalian brain is composed of many brain structures, each with its own ontogenetic and developmental history. We used single-nucleus RNA sequencing to sample over 2.4 million brain cells across 18 locations in the common marmoset, a New World monkey primed for genetic engineering, and examined gene expression patterns of cell types within and across brain structures. The adult transcriptomic identity of most neuronal types is shaped more by developmental origin than by neurotransmitter signaling repertoire. Quantitative mapping of GABAergic types with single-molecule FISH (smFISH) reveals that interneurons in the striatum and neocortex follow distinct spatial principles, and that lateral prefrontal and other higher-order cortical association areas are distinguished by high proportions of VIP+ neurons. We use cell type–specific enhancers to drive AAV-GFP and reconstruct the morphologies of molecularly resolved interneuron types in neocortex and striatum. Our analyses highlight how lineage, local context, and functional class contribute to the transcriptional identity and biodistribution of primate brain cell types.
AB - The mammalian brain is composed of many brain structures, each with its own ontogenetic and developmental history. We used single-nucleus RNA sequencing to sample over 2.4 million brain cells across 18 locations in the common marmoset, a New World monkey primed for genetic engineering, and examined gene expression patterns of cell types within and across brain structures. The adult transcriptomic identity of most neuronal types is shaped more by developmental origin than by neurotransmitter signaling repertoire. Quantitative mapping of GABAergic types with single-molecule FISH (smFISH) reveals that interneurons in the striatum and neocortex follow distinct spatial principles, and that lateral prefrontal and other higher-order cortical association areas are distinguished by high proportions of VIP+ neurons. We use cell type–specific enhancers to drive AAV-GFP and reconstruct the morphologies of molecularly resolved interneuron types in neocortex and striatum. Our analyses highlight how lineage, local context, and functional class contribute to the transcriptional identity and biodistribution of primate brain cell types.
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U2 - 10.1126/SCIADV.ADK3986
DO - 10.1126/SCIADV.ADK3986
M3 - Article
C2 - 37824615
AN - SCOPUS:85174752228
SN - 2375-2548
VL - 9
JO - Science Advances
JF - Science Advances
IS - 41
M1 - eadk3986
ER -