A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells

Jean Philippe Pellois; Tom W. Muir

doi:10.1002/anie.200501244

A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells

Jean Philippe Pellois, Tom W. Muir

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

(Graph Presented) Design and light: A photocleavable synthetic moiety is ligated to the C terminus of a recombinant protein. In the ligated state, the chemical and protein components influence the properties of one another. Upon irradiation with light, both elements are released and their respective function is recovered. In this way a protein can be delivered into living cells and activated by UV irradiation at a desired time or location.

Original language	English (US)
Pages (from-to)	5713-5717
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	35
DOIs	https://doi.org/10.1002/anie.200501244
State	Published - Sep 3 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Catalysis

Keywords

Bioorganic chemistry
Drug delivery
Photoactivation
Proteins

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10.1002/anie.200501244

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KW - Photoactivation

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A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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