A ligation and photorelease strategy for the temporal and spatial control of protein function in living cells

Jean Philippe Pellois, Tom W. Muir

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

(Graph Presented) Design and light: A photocleavable synthetic moiety is ligated to the C terminus of a recombinant protein. In the ligated state, the chemical and protein components influence the properties of one another. Upon irradiation with light, both elements are released and their respective function is recovered. In this way a protein can be delivered into living cells and activated by UV irradiation at a desired time or location.

Original languageEnglish (US)
Pages (from-to)5713-5717
Number of pages5
JournalAngewandte Chemie - International Edition
Volume44
Issue number35
DOIs
StatePublished - Sep 3 2005

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Keywords

  • Bioorganic chemistry
  • Drug delivery
  • Photoactivation
  • Proteins

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