A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A

Anamaris M. Colberg-Poley; Barrie J. Carter; Lynn W. Enquist

doi:10.1016/0378-1119(83)90093-8

A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A

Anamaris M. Colberg-Poley
, Barrie J. Carter
, Lynn W. Enquist

Research output: Contribution to journal › Article › peer-review

Abstract

Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.

Original language	English (US)
Pages (from-to)	327-330
Number of pages	4
Journal	Gene
Volume	24
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1119(83)90093-8
State	Published - Oct 1983

All Science Journal Classification (ASJC) codes

Genetics

Keywords

DNA polymorphism
Recombinant DNA
bacteriophage λ 1059 and λBF101
dot-blot hybridization
rapid mapping

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10.1016/0378-1119(83)90093-8

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title = "A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A",

abstract = "Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.",

keywords = "DNA polymorphism, Recombinant DNA, bacteriophage λ 1059 and λBF101, dot-blot hybridization, rapid mapping",

author = "Colberg-Poley, \{Anamaris M.\} and Carter, \{Barrie J.\} and Enquist, \{Lynn W.\}",

year = "1983",

month = oct,

doi = "10.1016/0378-1119(83)90093-8",

language = "English (US)",

volume = "24",

pages = "327--330",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

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T1 - A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A

AU - Colberg-Poley, Anamaris M.

AU - Carter, Barrie J.

AU - Enquist, Lynn W.

PY - 1983/10

Y1 - 1983/10

N2 - Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.

AB - Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.

KW - DNA polymorphism

KW - Recombinant DNA

KW - bacteriophage λ 1059 and λBF101

KW - dot-blot hybridization

KW - rapid mapping

UR - https://www.scopus.com/pages/publications/0020508253

UR - https://www.scopus.com/pages/publications/0020508253#tab=citedBy

U2 - 10.1016/0378-1119(83)90093-8

DO - 10.1016/0378-1119(83)90093-8

M3 - Article

C2 - 6315542

AN - SCOPUS:0020508253

SN - 0378-1119

VL - 24

SP - 327

EP - 330

JO - Gene

JF - Gene

IS - 2-3

ER -

A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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