A lambda library of Herpes simplex virus type 1 (KOS) DNA fragments obtained by partial digestion with Sau3A

Anamaris M. Colberg-Poley, Barrie J. Carter, Lynn W. Enquist

Research output: Contribution to journalArticlepeer-review

Abstract

Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalGene
Volume24
Issue number2-3
DOIs
StatePublished - Oct 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • DNA polymorphism
  • Recombinant DNA
  • bacteriophage λ 1059 and λBF101
  • dot-blot hybridization
  • rapid mapping

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