Abstract
Large fragments of Herpes simplex virus type 1 (HSV-1, strain KOS) DNA were produced by partial cleavage with Sau3A and inserted into a phage λ BamHI vector. Recombinant phage (λKOS) DNA molecules were isolated and characterised. The final collection of phage recombinants contains partially overlapping inserts, which represent most of the HSV-1 genome. Restriction enzyme analysis of many independent clones containing Us sequences revealed sequence polymorphism in two specific regions.
Original language | English (US) |
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Pages (from-to) | 327-330 |
Number of pages | 4 |
Journal | Gene |
Volume | 24 |
Issue number | 2-3 |
DOIs | |
State | Published - Oct 1983 |
All Science Journal Classification (ASJC) codes
- Genetics
Keywords
- DNA polymorphism
- Recombinant DNA
- bacteriophage λ 1059 and λBF101
- dot-blot hybridization
- rapid mapping