A hydrogen peroxide-responsive hyperpolarized 13C MRI contrast agent

Alexander R. Lippert; Kayvan R. Keshari; John Kurhanewicz; Christopher J. Chang

doi:10.1021/ja111589a

A hydrogen peroxide-responsive hyperpolarized ¹³C MRI contrast agent

Alexander R. Lippert, Kayvan R. Keshari, John Kurhanewicz, Christopher J. Chang

Research output: Contribution to journal › Article › peer-review

101 Scopus citations

Abstract

We report a new reaction-based approach for the detection of hydrogen peroxide (H₂O₂) using hyperpolarized ¹³C magnetic resonance imaging (¹³C MRI) and the H₂O ₂-mediated oxidation of α-ketoacids to carboxylic acids. ¹³C-Benzoylformic acid reacts selectively with H₂O ₂ over other reactive oxygen species to generate ¹³C- benzoic acid and can be hyperpolarized using dynamic nuclear polarization, providing a method for dual-frequency detection of H₂O₂. Phantom images collected using frequency-specific imaging sequences demonstrate the efficacy of this responsive contrast agent to monitor H₂O ₂ at pre-clinical field strengths. The combination of reaction-based detection chemistry and hyperpolarized ¹³C MRI provides a potentially powerful new methodology for non-invasive multi-analyte imaging in living systems.

Original language	English (US)
Pages (from-to)	3776-3779
Number of pages	4
Journal	Journal of the American Chemical Society
Volume	133
Issue number	11
DOIs	https://doi.org/10.1021/ja111589a
State	Published - Mar 23 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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title = "A hydrogen peroxide-responsive hyperpolarized 13C MRI contrast agent",

abstract = "We report a new reaction-based approach for the detection of hydrogen peroxide (H2O2) using hyperpolarized 13C magnetic resonance imaging (13C MRI) and the H2O 2-mediated oxidation of α-ketoacids to carboxylic acids. 13C-Benzoylformic acid reacts selectively with H2O 2 over other reactive oxygen species to generate 13C- benzoic acid and can be hyperpolarized using dynamic nuclear polarization, providing a method for dual-frequency detection of H2O2. Phantom images collected using frequency-specific imaging sequences demonstrate the efficacy of this responsive contrast agent to monitor H2O 2 at pre-clinical field strengths. The combination of reaction-based detection chemistry and hyperpolarized 13C MRI provides a potentially powerful new methodology for non-invasive multi-analyte imaging in living systems.",

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T1 - A hydrogen peroxide-responsive hyperpolarized 13C MRI contrast agent

AU - Lippert, Alexander R.

AU - Keshari, Kayvan R.

AU - Kurhanewicz, John

AU - Chang, Christopher J.

PY - 2011/3/23

Y1 - 2011/3/23

N2 - We report a new reaction-based approach for the detection of hydrogen peroxide (H2O2) using hyperpolarized 13C magnetic resonance imaging (13C MRI) and the H2O 2-mediated oxidation of α-ketoacids to carboxylic acids. 13C-Benzoylformic acid reacts selectively with H2O 2 over other reactive oxygen species to generate 13C- benzoic acid and can be hyperpolarized using dynamic nuclear polarization, providing a method for dual-frequency detection of H2O2. Phantom images collected using frequency-specific imaging sequences demonstrate the efficacy of this responsive contrast agent to monitor H2O 2 at pre-clinical field strengths. The combination of reaction-based detection chemistry and hyperpolarized 13C MRI provides a potentially powerful new methodology for non-invasive multi-analyte imaging in living systems.

AB - We report a new reaction-based approach for the detection of hydrogen peroxide (H2O2) using hyperpolarized 13C magnetic resonance imaging (13C MRI) and the H2O 2-mediated oxidation of α-ketoacids to carboxylic acids. 13C-Benzoylformic acid reacts selectively with H2O 2 over other reactive oxygen species to generate 13C- benzoic acid and can be hyperpolarized using dynamic nuclear polarization, providing a method for dual-frequency detection of H2O2. Phantom images collected using frequency-specific imaging sequences demonstrate the efficacy of this responsive contrast agent to monitor H2O 2 at pre-clinical field strengths. The combination of reaction-based detection chemistry and hyperpolarized 13C MRI provides a potentially powerful new methodology for non-invasive multi-analyte imaging in living systems.

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A hydrogen peroxide-responsive hyperpolarized ¹³C MRI contrast agent

Abstract

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