A high melting structure in DNA distinguishes phases of the cell cycle

Gretchen A. Rice, Nancy A. Touchette, Angela N.H. Creager, Jonathan Goldberg, R. David Cole

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3 Scopus citations


Differential scanning microcalorimetry of the nuclei of dividing CHO cells revealed DNA structures that showed structural transitions at 60, 76, 88, and 105 °C (transitions I to IV, respectively). In cultures synchronized by isoleucine deprivation the enthalpies of transitions I and II were rather constant throughout the cell cycle. While the sum of the enthalpies of III and IV was nearly constant, the ratio of IV to III varied substantially from one phase of the cycle to another. A high IV:III ratio of 6 characterized G1 while S phase gave a IV:III ratio of about 2. Cells containing metaphase chromosomes also showed a IV:III ratio near 2. The IV:III ratio for CHO cells showed a progressive decrease as the cells were maintained in isoleucine-free medium from 0 to 6 days.

Original languageEnglish (US)
Pages (from-to)221-226
Number of pages6
JournalExperimental Cell Research
Issue number1
StatePublished - Jul 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology


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