TY - JOUR
T1 - A genetic link between morphogenesis and cell division during formation of the ventral furrow in Drosophila
AU - Großhans, Jörg
AU - Wieschaus, Eric
N1 - Funding Information:
We thank A. Müller for the initial identification of the gastrulation phenotype of the 71 and 77 regions, T. Seher and M. Leptin for discussion and sharing of unpublished data, and the members of the Wieschaus and Schüpbach laboratories for helpful discussions. We thank J. Goodhouse for assistance with confocal microscopy, C. Hsuan-Hung for maintaining the laboratory stock collection. We gratefully received reagents, materials, and stocks from D. Andrew, C. Avides, K. Barrett, L. Cherbas, D. DeSousa, B. Edgar, M. Fortini, B. Ganetzky, U. Häcker, T. Hummel, T. Ip, N. Ito, K. Irvine, D. Kalderon, I. Koch, T. Laverty, K. Lunde, S. Luschnig, U. Nauber, N. Perrimon, J. Reinitz, S. Roth, R. Rupp, K. Sauer, F. Schnorrer, T. Seher, S. Sigrist, S. Smolik, A. Spradling, F. Sprenger, D. St Johnston, H. Stocker, and C. Wood. We received fly stocks from the centers in Berkeley, Bloomington, Bowling Green, Umeå, and Szeged. R. Hoang and C. Brachthäuser were so kind to read and improve the manuscript. J. G. was supported by a long-term fellowship of the Human Science Frontier Organisation and acknowledges financial aid from his parents to complement the salary by HHMI. This work was supported by the Howard Hughes Medical Institute and grant 5R37HD15587 from the NICHD.
PY - 2000/5/26
Y1 - 2000/5/26
N2 - Stages in development with rapid transitions between mitosis and morphogenesis may require specific mechanisms to coordinate cell shape change. Here we describe a novel mitotic inhibitor that acts during Drosophila gastrulation to counteract String/Cdc25, specifically in the cells that invaginate to form the mesoderm. We have identified two genes, fruhstart and tribbles, that are required for this ventral inhibition. tribbles encodes a kinase-related protein whose RNA, however, is also present outside of the ventral region. Effective inhibition of mitosis in the cells of the ventral furrow depends on the transcription factor Snail that triggers the ventral cell shape changes. When overexpressed in a microinjection assay, Tribbles directly inhibits mitosis. We propose that Fruhstart and Tribbles form a link between the morphogenetic movements and mitotic control.
AB - Stages in development with rapid transitions between mitosis and morphogenesis may require specific mechanisms to coordinate cell shape change. Here we describe a novel mitotic inhibitor that acts during Drosophila gastrulation to counteract String/Cdc25, specifically in the cells that invaginate to form the mesoderm. We have identified two genes, fruhstart and tribbles, that are required for this ventral inhibition. tribbles encodes a kinase-related protein whose RNA, however, is also present outside of the ventral region. Effective inhibition of mitosis in the cells of the ventral furrow depends on the transcription factor Snail that triggers the ventral cell shape changes. When overexpressed in a microinjection assay, Tribbles directly inhibits mitosis. We propose that Fruhstart and Tribbles form a link between the morphogenetic movements and mitotic control.
UR - http://www.scopus.com/inward/record.url?scp=0034716888&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034716888&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80862-4
DO - 10.1016/S0092-8674(00)80862-4
M3 - Article
C2 - 10850494
AN - SCOPUS:0034716888
VL - 101
SP - 523
EP - 531
JO - Cell
JF - Cell
SN - 0092-8674
IS - 5
ER -