Abstract
Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1167-1174 |
| Number of pages | 8 |
| Journal | EMBO Reports |
| Volume | 12 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 2011 |
All Science Journal Classification (ASJC) codes
- Genetics
- Molecular Biology
- Biochemistry
Keywords
- Drosophila tracheal development
- EP screen
- MS2-MCP system
- RNA tagging
- mRNA localization
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