A genetic in vivo system to detect asymmetrically distributed RNA

N. Jayanandanan, Elizabeth R. Gavis, Veit Riechmann, Maria Leptin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.

Original languageEnglish (US)
Pages (from-to)1167-1174
Number of pages8
JournalEMBO Reports
Volume12
Issue number11
DOIs
StatePublished - Nov 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Keywords

  • Drosophila tracheal development
  • EP screen
  • MS2-MCP system
  • RNA tagging
  • mRNA localization

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