A genetic in vivo system to detect asymmetrically distributed RNA

N. Jayanandanan; Elizabeth R. Gavis; Veit Riechmann; Maria Leptin

doi:10.1038/embor.2011.178

A genetic in vivo system to detect asymmetrically distributed RNA

N. Jayanandanan, Elizabeth R. Gavis, Veit Riechmann, Maria Leptin

Molecular Biology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.

Original language	English (US)
Pages (from-to)	1167-1174
Number of pages	8
Journal	EMBO Reports
Volume	12
Issue number	11
DOIs	https://doi.org/10.1038/embor.2011.178
State	Published - Nov 2011

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology
Biochemistry

Keywords

Drosophila tracheal development
EP screen
MS2-MCP system
RNA tagging
mRNA localization

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10.1038/embor.2011.178

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AB - Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.

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KW - RNA tagging

KW - mRNA localization

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A genetic in vivo system to detect asymmetrically distributed RNA

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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