A flexible protein linker improves the function of epitope-tagged proteins in Saccharomyces cerevisiae

Michelle Sabourin, Creighton T. Tuzon, Timothy S. Fisher, Virginia A. Zakian

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Epitope tagging permits the detection of proteins when protein-specific antibodies are not available. However, the epitope tag can reduce the function of the tagged protein. Here we describe a cassette that can be used to introduce an eight amino acid flexible linker between multiple Myc epitopes and the open reading frame of a given gene. We show that inserting the linker improves the in vivo ability of the telomerase subunits Est2p and Est1p to maintain telomere length. The methods used here are generally applicable to improve the function of tagged proteins in both Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalYeast
Volume24
Issue number1
DOIs
StatePublished - Jan 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Genetics

Keywords

  • Epitope tag
  • In vivo function
  • Protein linker
  • Telomere
  • Yeast telomerase

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