A Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning

Gerard Joey Broussard, Mikhail Kislin, Caroline Jung, Samuel S.H. Wang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Climbing fiber inputs to Purkinje cells provide instructive signals critical for cerebellum-dependent associative learning. Studying these signals in head-fixed mice facilitates the use of imaging, electrophysiological, and optogenetic methods. Here, a low-cost behavioral platform (~$1000) was developed that allows tracking of associative learning in head-fixed mice that locomote freely on a running wheel. The platform incorporates two common associative learning paradigms: eyeblink conditioning and delayed tactile startle conditioning. Behavior is tracked using a camera and the wheel movement by a detector. We describe the components and setup and provide a detailed protocol for training and data analysis. This platform allows the incorporation of optogenetic stimulation and fluorescence imaging. The design allows a single host computer to control multiple platforms for training multiple animals simultaneously.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number179
DOIs
StatePublished - Jan 19 2022

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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