A dynamically assembled cell wall synthesis machinery buffers cell growth

Timothy K. Lee, Carolina Tropini, Jen Hsin, Samantha M. Desmarais, Tristan S. Ursell, Enhao Gong, Zemer Gitai, Russell D. Monds, Kerwyn Casey Huang

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

Assembly of protein complexes is a key mechanism for achieving spatial and temporal coordination in processes involving many enzymes. Growth of rod-shaped bacteria is a well-studied example requiring such coordination; expansion of the cell wall is thought to involve coordination of the activity of synthetic enzymes with the cytoskeleton via a stable complex. Here, we use single-molecule tracking to demonstrate that the bacterial actin homolog MreB and the essential cell wall enzyme PBP2 move on timescales orders of magnitude apart, with drastically different characteristic motions. Our observations suggest that PBP2 interacts with the rest of the synthesis machinery through a dynamic cycle of transient association. Consistent with this model, growth is robust to large fluctuations in PBP2 abundance. In contrast to stable complex formation, dynamic association of PBP2 is less dependent on the function of other components of the synthesis machinery, and buffers spatially distributed growth against fluctuations in pathway component concentrations and the presence of defective components. Dynamic association could generally represent an efficient strategy for spatiotemporal coordination of protein activities, especially when excess concentrations of system components are inhibitory to the overall process or deleterious to the cell.

Original languageEnglish (US)
Pages (from-to)4554-4559
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number12
DOIs
StatePublished - Mar 25 2014

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Bacterial cell wall
  • Multienzyme complexes
  • Pencillin binding proteins
  • Superresolution microscopy

Fingerprint Dive into the research topics of 'A dynamically assembled cell wall synthesis machinery buffers cell growth'. Together they form a unique fingerprint.

Cite this