A Drosophila melanogaster transfer RNA gene cluster at the cytogenetic locus 90BC

Robert DeLotto; Paul Schedl

doi:10.1016/0022-2836(84)90157-8

A Drosophila melanogaster transfer RNA gene cluster at the cytogenetic locus 90BC

Robert DeLotto, Paul Schedl

Molecular Biology

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Abstract

We report the isolation and characterization of a 31 × 10³ base-pair DNA segment containing a cluster of Drosophila melanogaster transfer RNA genes from the oytogenic locus 90BC. Seven distinct coding regions have been identified in a 15 × 10³ base-pair DNA segment. These coding regions contain at least ten tRNA structural genes and include sequences encoding the following tRNAs: tRNA^Val, tRNA^Pro, tRNA^Ala and tRNA^Thr. We have determined the nucleotide sequence of six of these structural genes and their flanking regions. These genes do not contain intervening sequences nor do they encode the terminal CCA. The tRNA genes from the locus also appear to be functional when assayed in a Xenopus germinal vesicle in vitro transcriptional system.

Original language	English (US)
Pages (from-to)	587-605
Number of pages	19
Journal	Journal of Molecular Biology
Volume	179
Issue number	4
DOIs	https://doi.org/10.1016/0022-2836(84)90157-8
State	Published - Nov 15 1984

All Science Journal Classification (ASJC) codes

Molecular Biology
Structural Biology

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10.1016/0022-2836(84)90157-8

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abstract = "We report the isolation and characterization of a 31 × 103 base-pair DNA segment containing a cluster of Drosophila melanogaster transfer RNA genes from the oytogenic locus 90BC. Seven distinct coding regions have been identified in a 15 × 103 base-pair DNA segment. These coding regions contain at least ten tRNA structural genes and include sequences encoding the following tRNAs: tRNAVal, tRNAPro, tRNAAla and tRNAThr. We have determined the nucleotide sequence of six of these structural genes and their flanking regions. These genes do not contain intervening sequences nor do they encode the terminal CCA. The tRNA genes from the locus also appear to be functional when assayed in a Xenopus germinal vesicle in vitro transcriptional system.",

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AB - We report the isolation and characterization of a 31 × 103 base-pair DNA segment containing a cluster of Drosophila melanogaster transfer RNA genes from the oytogenic locus 90BC. Seven distinct coding regions have been identified in a 15 × 103 base-pair DNA segment. These coding regions contain at least ten tRNA structural genes and include sequences encoding the following tRNAs: tRNAVal, tRNAPro, tRNAAla and tRNAThr. We have determined the nucleotide sequence of six of these structural genes and their flanking regions. These genes do not contain intervening sequences nor do they encode the terminal CCA. The tRNA genes from the locus also appear to be functional when assayed in a Xenopus germinal vesicle in vitro transcriptional system.

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A Drosophila melanogaster transfer RNA gene cluster at the cytogenetic locus 90BC

Abstract

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