A Distance-Weighted Interaction Map Reveals a Previously Uncharacterized Layer of the Bacillus subtilis Spore Coat

Peter T. McKenney, Adam Driks, Haig A. Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H. Wang, Zemer Gitai, Patrick Eichenberger

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Bacillus subtilis spores are encased in a protein assembly called the spore coat that is made up of at least 70 different proteins. Conventional electron microscopy shows the coat to be organized into two distinct layers. Because the coat is about as wide as the theoretical limit of light microscopy, quantitatively measuring the localization of individual coat proteins within the coat is challenging. We used fusions of coat proteins to green fluorescent protein to map genetic dependencies for coat assembly and to define three independent subnetworks of coat proteins. To complement the genetic data, we measured coat protein localization at subpixel resolution and integrated these two data sets to produce a distance-weighted genetic interaction map. Using these data, we predict that the coat comprises at least four spatially distinct layers, including a previously uncharacterized glycoprotein outermost layer that we name the spore crust. We found that crust assembly depends on proteins we predicted to localize to the crust. The crust may be conserved in all Bacillus spores and may play critical functions in the environment.

Original languageEnglish (US)
Pages (from-to)934-938
Number of pages5
JournalCurrent Biology
Volume20
Issue number10
DOIs
StatePublished - May 25 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    McKenney, P. T., Driks, A., Eskandarian, H. A., Grabowski, P., Guberman, J., Wang, K. H., Gitai, Z., & Eichenberger, P. (2010). A Distance-Weighted Interaction Map Reveals a Previously Uncharacterized Layer of the Bacillus subtilis Spore Coat. Current Biology, 20(10), 934-938. https://doi.org/10.1016/j.cub.2010.03.060