A deletion analysis of lambda hybrid phage carrying the Us region of Herpes simplex virus type 1 (Patton) II. Construction of an SmaI map

K. Umene, L. W. Enquist

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The 15.4 kb EcoRI-H fragment of Herpes simplex virus type 1 (HSV-1) strain Patton, which contains the entire short unique (Us) region, has been cloned in bacteriophage λ. The fragment contains a terminal redundancy of about 900 bp that represents the S region terminal-repeat sequences. The restriction enzyme SmaI cleaves the EcoRI-H fragment at more than 30 sites. We have constructed an SmaI map of this fragment using thirteen isolates of λgtWES hybrid bacteriophage that carry various deletions of the EcoRI-H fragment.

Original languageEnglish (US)
Pages (from-to)269-279
Number of pages11
JournalGene
Volume13
Issue number3
DOIs
StatePublished - Apr 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Recombinant DNA
  • srestriction and deletion mapping
  • λgtWES vector

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