The 15.4 kb EcoRI-H fragment of Herpes simplex virus type 1 (HSV-1) strain Patton, which contains the entire short unique (Us) region, has been cloned in bacteriophage λ. The fragment contains a terminal redundancy of about 900 bp that represents the S region terminal-repeat sequences. The restriction enzyme SmaI cleaves the EcoRI-H fragment at more than 30 sites. We have constructed an SmaI map of this fragment using thirteen isolates of λgtWES hybrid bacteriophage that carry various deletions of the EcoRI-H fragment.
All Science Journal Classification (ASJC) codes
- Recombinant DNA
- srestriction and deletion mapping
- λgtWES vector