A deletion analysis of lambda hybrid phage carrying the Us region of Herpes simplex virus type 1 (Patton) II. Construction of an SmaI map

K. Umene; L. W. Enquist

doi:10.1016/0378-1119(81)90031-7

A deletion analysis of lambda hybrid phage carrying the U_s region of Herpes simplex virus type 1 (Patton) II. Construction of an SmaI map

K. Umene, L. W. Enquist

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The 15.4 kb EcoRI-H fragment of Herpes simplex virus type 1 (HSV-1) strain Patton, which contains the entire short unique (U_s) region, has been cloned in bacteriophage λ. The fragment contains a terminal redundancy of about 900 bp that represents the S region terminal-repeat sequences. The restriction enzyme SmaI cleaves the EcoRI-H fragment at more than 30 sites. We have constructed an SmaI map of this fragment using thirteen isolates of λgtWES hybrid bacteriophage that carry various deletions of the EcoRI-H fragment.

Original language	English (US)
Pages (from-to)	269-279
Number of pages	11
Journal	Gene
Volume	13
Issue number	3
DOIs	https://doi.org/10.1016/0378-1119(81)90031-7
State	Published - Apr 1981

All Science Journal Classification (ASJC) codes

Genetics

Keywords

Recombinant DNA
srestriction and deletion mapping
λgtWES vector

Access to Document

10.1016/0378-1119(81)90031-7

Cite this

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title = "A deletion analysis of lambda hybrid phage carrying the Us region of Herpes simplex virus type 1 (Patton) II. Construction of an SmaI map",

abstract = "The 15.4 kb EcoRI-H fragment of Herpes simplex virus type 1 (HSV-1) strain Patton, which contains the entire short unique (Us) region, has been cloned in bacteriophage λ. The fragment contains a terminal redundancy of about 900 bp that represents the S region terminal-repeat sequences. The restriction enzyme SmaI cleaves the EcoRI-H fragment at more than 30 sites. We have constructed an SmaI map of this fragment using thirteen isolates of λgtWES hybrid bacteriophage that carry various deletions of the EcoRI-H fragment.",

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note = "Funding Information: Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205 (U.S.A.)",

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T1 - A deletion analysis of lambda hybrid phage carrying the Us region of Herpes simplex virus type 1 (Patton) II. Construction of an SmaI map

AU - Umene, K.

AU - Enquist, L. W.

N1 - Funding Information: Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20205 (U.S.A.)

PY - 1981/4

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AB - The 15.4 kb EcoRI-H fragment of Herpes simplex virus type 1 (HSV-1) strain Patton, which contains the entire short unique (Us) region, has been cloned in bacteriophage λ. The fragment contains a terminal redundancy of about 900 bp that represents the S region terminal-repeat sequences. The restriction enzyme SmaI cleaves the EcoRI-H fragment at more than 30 sites. We have constructed an SmaI map of this fragment using thirteen isolates of λgtWES hybrid bacteriophage that carry various deletions of the EcoRI-H fragment.

KW - Recombinant DNA

KW - srestriction and deletion mapping

KW - λgtWES vector

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