@article{dff74be494dd49ad877aa97f97a8d768,
title = "A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli",
abstract = "Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.",
author = "Donnelly, {Ann E.} and Murphy, {Grant S.} and Digianantonio, {Katherine M.} and Hecht, {Michael H.}",
note = "Funding Information: We thank J. Eng for assistance with LC–MS and E. Nolan (MIT) for providing D-enterobactin. This work was funded by NSF Grant MCB-1409402, and by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program to A.E.D. Funding Information: We thank J. Eng for assistance with LCMS and E. Nolan (MIT) for providing D-enterobactin. This work was funded by NSF Grant MCB-1409402, and by the Department of Defense (DoD) through the National Defense Science & Engineering Graduate Fellowship (NDSEG) Program to A.E.D.",
year = "2018",
month = mar,
day = "1",
doi = "10.1038/nchembio.2550",
language = "English (US)",
volume = "14",
pages = "253--255",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "3",
}