A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli

Ann E. Donnelly; Grant S. Murphy; Katherine M. Digianantonio; Michael H. Hecht

doi:10.1038/nchembio.2550

A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli

Ann E. Donnelly, Grant S. Murphy, Katherine M. Digianantonio, Michael H. Hecht

Research output: Contribution to journal › Article

21 Scopus citations

Abstract

Producing novel enzymes that are catalytically active in vitro and biologically functional in vivo is a key goal of synthetic biology. Here we describe Syn-F4, the first de novo protein that meets both criteria. Purified Syn-F4 hydrolyzes the siderophore ferric enterobactin, and expression of Syn-F4 allows an inviable strain of Escherichia coli to grow in iron-limited medium. These findings demonstrate that entirely new sequences can provide life-sustaining enzymatic functions in living organisms.

Original language	English (US)
Pages (from-to)	253-255
Number of pages	3
Journal	Nature Chemical Biology
Volume	14
Issue number	3
DOIs	https://doi.org/10.1038/nchembio.2550
State	Published - Mar 1 2018

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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10.1038/nchembio.2550

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Donnelly, A. E., Murphy, G. S., Digianantonio, K. M., & Hecht, M. H. (2018). A de novo enzyme catalyzes a life-sustaining reaction in Escherichia coli. Nature Chemical Biology, 14(3), 253-255. https://doi.org/10.1038/nchembio.2550

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