TY - JOUR
T1 - A Click-Chemistry-Based Enrichable Crosslinker for Structural and Protein Interaction Analysis by Mass Spectrometry
AU - Stadlmeier, Michael
AU - Runtsch, Leander Simon
AU - Streshnev, Filipp
AU - Wühr, Martin
AU - Carell, Thomas
N1 - Publisher Copyright:
© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
PY - 2020/1/15
Y1 - 2020/1/15
N2 - Mass spectrometry is the method of choice for the characterisation of proteomes. Most proteins operate in protein complexes, in which their close association modulates their function. However, with standard MS analysis, information on protein–protein interactions is lost and no structural information is retained. To gain structural and interactome data, new crosslinking reagents are needed that freeze inter- and intramolecular interactions. Herein, the development of a new reagent, which has several features that enable highly sensitive crosslinking MS, is reported. The reagent enables enrichment of crosslinked peptides from the majority of background peptides to facilitate efficient detection of low-abundant crosslinked peptides. Due to the special cleavable properties, the reagent can be used for MS2 and potentially for MS3 experiments. Thus, the new crosslinking reagent, in combination with high-end MS, should enable sensitive analysis of interactomes, which will help researchers to obtain important insights into cellular states in health and diseases.
AB - Mass spectrometry is the method of choice for the characterisation of proteomes. Most proteins operate in protein complexes, in which their close association modulates their function. However, with standard MS analysis, information on protein–protein interactions is lost and no structural information is retained. To gain structural and interactome data, new crosslinking reagents are needed that freeze inter- and intramolecular interactions. Herein, the development of a new reagent, which has several features that enable highly sensitive crosslinking MS, is reported. The reagent enables enrichment of crosslinked peptides from the majority of background peptides to facilitate efficient detection of low-abundant crosslinked peptides. Due to the special cleavable properties, the reagent can be used for MS2 and potentially for MS3 experiments. Thus, the new crosslinking reagent, in combination with high-end MS, should enable sensitive analysis of interactomes, which will help researchers to obtain important insights into cellular states in health and diseases.
KW - click chemistry
KW - crosslinking mass spectrometry
KW - interactome analysis
KW - proteomics
KW - structure elucidation
UR - http://www.scopus.com/inward/record.url?scp=85075738869&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075738869&partnerID=8YFLogxK
U2 - 10.1002/cbic.201900611
DO - 10.1002/cbic.201900611
M3 - Article
C2 - 31593346
AN - SCOPUS:85075738869
SN - 1439-4227
VL - 21
SP - 103
EP - 107
JO - ChemBioChem
JF - ChemBioChem
IS - 1-2
ER -