A cellular platform for production of C4 monomers

Matthew A. Davis; Vivian Yaci Yu; Beverly Fu; Miao Wen; Edward J. Koleski; Joshua Silverman; Charles A. Berdan; Daniel K. Nomura; Michelle C.Y. Chang

doi:10.1039/d3sc02773b

A cellular platform for production of C₄ monomers

Matthew A. Davis, Vivian Yaci Yu, Beverly Fu, Miao Wen, Edward J. Koleski, Joshua Silverman, Charles A. Berdan, Daniel K. Nomura, Michelle C.Y. Chang

Research output: Contribution to journal › Article › peer-review

Abstract

Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved yields of up to ∼95% for three C₄ commodity chemicals, n-butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points.

Original language	English (US)
Pages (from-to)	11718-11726
Number of pages	9
Journal	Chemical Science
Volume	14
Issue number	42
DOIs	https://doi.org/10.1039/d3sc02773b
State	Published - Oct 9 2023
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry

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10.1039/d3sc02773b

Cite this

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title = "A cellular platform for production of C4 monomers",

abstract = "Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved yields of up to ∼95% for three C4 commodity chemicals, n-butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points.",

author = "Davis, {Matthew A.} and Yu, {Vivian Yaci} and Beverly Fu and Miao Wen and Koleski, {Edward J.} and Joshua Silverman and Berdan, {Charles A.} and Nomura, {Daniel K.} and Chang, {Michelle C.Y.}",

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AU - Davis, Matthew A.

AU - Yu, Vivian Yaci

AU - Fu, Beverly

AU - Wen, Miao

AU - Koleski, Edward J.

AU - Silverman, Joshua

AU - Berdan, Charles A.

AU - Nomura, Daniel K.

AU - Chang, Michelle C.Y.

PY - 2023/10/9

Y1 - 2023/10/9

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AB - Living organisms carry out a wide range of remarkable functions, including the synthesis of thousands of simple and complex chemical structures for cellular growth and maintenance. The manipulation of this reaction network has allowed for the genetic engineering of cells for targeted chemical synthesis, but it remains challenging to alter the program underlying their fundamental chemical behavior. By taking advantage of the unique ability of living systems to use evolution to find solutions to complex problems, we have achieved yields of up to ∼95% for three C4 commodity chemicals, n-butanol, 1,3-butanediol, and 4-hydroxy-2-butanone. Genomic sequencing of the evolved strains identified pcnB and rpoBC as two gene loci that are able to alter carbon flow by remodeling the transcriptional landscape of the cell, highlighting the potential of synthetic pathways as a tool to identify metabolic control points.

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A cellular platform for production of C₄ monomers

Abstract

All Science Journal Classification (ASJC) codes

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