A cell-assembled, spatially aligned extracellular matrix to promote directed tissue development

A nanometer thick, micron scale-patterned interface on a polymeric material directs fibroblast proliferation into a highly aligned, confluent cell monolayer. These cells assemble fibronectin extracellular matrix (ECM) fibrils that are aligned with the pattern, and matrix alignment on the synthetic polymer surface is maintained throughout a decellularization process. Biologic relevance of this ECM-synthetic material composite is illustrated by directing oriented neurite outgrowth in register with the aligned matrix fibrils.