A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater

Daniel Mikhail Sigman; K. L. Casciotti; M. Andreani; C. Barford; M. Galanter; J. K. Böhlke

doi:10.1021/ac010088e

A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater

Daniel Mikhail Sigman, K. L. Casciotti, M. Andreani, C. Barford, M. Galanter, J. K. Böhlke

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Abstract

We report a new method for measurement of the isotopic composition of nitrate (NO₃^-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N₂O) generated from nitrate by denitrifying bacteria that lack N₂O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2‰ (1 SD) at concentrations of nitrate down to 1 μM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 μM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.

Original language	English (US)
Pages (from-to)	4145-4153
Number of pages	9
Journal	Analytical Chemistry
Volume	73
Issue number	17
DOIs	https://doi.org/10.1021/ac010088e
State	Published - Sep 1 2001

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac010088e

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title = "A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater",

abstract = "We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2‰ (1 SD) at concentrations of nitrate down to 1 μM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 μM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.",

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T1 - A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater

AU - Sigman, Daniel Mikhail

AU - Casciotti, K. L.

AU - Andreani, M.

AU - Barford, C.

AU - Galanter, M.

AU - Böhlke, J. K.

PY - 2001/9/1

Y1 - 2001/9/1

N2 - We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2‰ (1 SD) at concentrations of nitrate down to 1 μM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 μM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.

AB - We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater. The method is based on the isotopic analysis of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria that lack N2O-reductase activity. The isotopic composition of both nitrogen and oxygen from nitrate are accessible in this way. In this first of two companion manuscripts, we describe the basic protocol and results for the nitrogen isotopes. The precision of the method is better than 0.2‰ (1 SD) at concentrations of nitrate down to 1 μM, and the nitrogen isotopic differences among various standards and samples are accurately reproduced. For samples with 1 μM nitrate or more, the blank of the method is less than 10% of the signal size, and various approaches may reduce it further.

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A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater

Abstract

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