A bacterial antibiotic resistance accelerator and applications

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The systematic emergence of drug resistance remains a major problem in the treatment of infectious diseases (antibiotics) and cancer (chemotherapy), with possible common fundamental origins linking bacterial antibiotic resistance and emergence of chemotherapy resistance. The common link may be evolution in a complex fitness landscape with connected small population niches. We report a detailed method for observing bacterial adaptive behavior in heterogeneous microfluidic environment designed to mimic the environmental heterogeneity found in natural microbial niches. First, the device is structured with multiple connected micro-chambers that allow the cell population to communicate and organize into smaller populations. Second, bacteria evolve within an antibiotic gradient generated throughout the micro-chambers that creates a wide range of fitness landscapes. High-resolution images of the adaptive response to the antibiotic stress are captured by epifluorescence microscopy at various levels of the bacterial organization for quantitative analysis. Thus, the experimental setup we have developed provides a powerful frame for visualizing evolution at work: bacterial movement, survival and death. It also presents a basis for exploring the rates at which drug resistance arises in bacteria and other biological contexts such as cancer.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
EditorsMatthieu Piel, Daniel Fletcher, Junsang Doh
PublisherAcademic Press Inc.
Pages41-57
Number of pages17
ISBN (Print)9780128142820
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Cell Biology
Volume147
ISSN (Print)0091-679X

Fingerprint

Bacterial Drug Resistance
Anti-Bacterial Agents
Drug Resistance
Population
Bacteria
Drug Therapy
Microfluidics
Psychological Adaptation
Communicable Diseases
Microscopy
Neoplasms
Equipment and Supplies
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Bos, J., & Austin, R. H. (2018). A bacterial antibiotic resistance accelerator and applications. In M. Piel, D. Fletcher, & J. Doh (Eds.), Methods in Cell Biology (pp. 41-57). (Methods in Cell Biology; Vol. 147). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2018.06.005
Bos, Julia ; Austin, Robert Hamilton. / A bacterial antibiotic resistance accelerator and applications. Methods in Cell Biology. editor / Matthieu Piel ; Daniel Fletcher ; Junsang Doh. Academic Press Inc., 2018. pp. 41-57 (Methods in Cell Biology).
@inbook{54bee5de4c3e4b46bac07138334642f7,
title = "A bacterial antibiotic resistance accelerator and applications",
abstract = "The systematic emergence of drug resistance remains a major problem in the treatment of infectious diseases (antibiotics) and cancer (chemotherapy), with possible common fundamental origins linking bacterial antibiotic resistance and emergence of chemotherapy resistance. The common link may be evolution in a complex fitness landscape with connected small population niches. We report a detailed method for observing bacterial adaptive behavior in heterogeneous microfluidic environment designed to mimic the environmental heterogeneity found in natural microbial niches. First, the device is structured with multiple connected micro-chambers that allow the cell population to communicate and organize into smaller populations. Second, bacteria evolve within an antibiotic gradient generated throughout the micro-chambers that creates a wide range of fitness landscapes. High-resolution images of the adaptive response to the antibiotic stress are captured by epifluorescence microscopy at various levels of the bacterial organization for quantitative analysis. Thus, the experimental setup we have developed provides a powerful frame for visualizing evolution at work: bacterial movement, survival and death. It also presents a basis for exploring the rates at which drug resistance arises in bacteria and other biological contexts such as cancer.",
author = "Julia Bos and Austin, {Robert Hamilton}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/bs.mcb.2018.06.005",
language = "English (US)",
isbn = "9780128142820",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "41--57",
editor = "Matthieu Piel and Daniel Fletcher and Junsang Doh",
booktitle = "Methods in Cell Biology",
address = "United States",

}

Bos, J & Austin, RH 2018, A bacterial antibiotic resistance accelerator and applications. in M Piel, D Fletcher & J Doh (eds), Methods in Cell Biology. Methods in Cell Biology, vol. 147, Academic Press Inc., pp. 41-57. https://doi.org/10.1016/bs.mcb.2018.06.005

A bacterial antibiotic resistance accelerator and applications. / Bos, Julia; Austin, Robert Hamilton.

Methods in Cell Biology. ed. / Matthieu Piel; Daniel Fletcher; Junsang Doh. Academic Press Inc., 2018. p. 41-57 (Methods in Cell Biology; Vol. 147).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - A bacterial antibiotic resistance accelerator and applications

AU - Bos, Julia

AU - Austin, Robert Hamilton

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The systematic emergence of drug resistance remains a major problem in the treatment of infectious diseases (antibiotics) and cancer (chemotherapy), with possible common fundamental origins linking bacterial antibiotic resistance and emergence of chemotherapy resistance. The common link may be evolution in a complex fitness landscape with connected small population niches. We report a detailed method for observing bacterial adaptive behavior in heterogeneous microfluidic environment designed to mimic the environmental heterogeneity found in natural microbial niches. First, the device is structured with multiple connected micro-chambers that allow the cell population to communicate and organize into smaller populations. Second, bacteria evolve within an antibiotic gradient generated throughout the micro-chambers that creates a wide range of fitness landscapes. High-resolution images of the adaptive response to the antibiotic stress are captured by epifluorescence microscopy at various levels of the bacterial organization for quantitative analysis. Thus, the experimental setup we have developed provides a powerful frame for visualizing evolution at work: bacterial movement, survival and death. It also presents a basis for exploring the rates at which drug resistance arises in bacteria and other biological contexts such as cancer.

AB - The systematic emergence of drug resistance remains a major problem in the treatment of infectious diseases (antibiotics) and cancer (chemotherapy), with possible common fundamental origins linking bacterial antibiotic resistance and emergence of chemotherapy resistance. The common link may be evolution in a complex fitness landscape with connected small population niches. We report a detailed method for observing bacterial adaptive behavior in heterogeneous microfluidic environment designed to mimic the environmental heterogeneity found in natural microbial niches. First, the device is structured with multiple connected micro-chambers that allow the cell population to communicate and organize into smaller populations. Second, bacteria evolve within an antibiotic gradient generated throughout the micro-chambers that creates a wide range of fitness landscapes. High-resolution images of the adaptive response to the antibiotic stress are captured by epifluorescence microscopy at various levels of the bacterial organization for quantitative analysis. Thus, the experimental setup we have developed provides a powerful frame for visualizing evolution at work: bacterial movement, survival and death. It also presents a basis for exploring the rates at which drug resistance arises in bacteria and other biological contexts such as cancer.

UR - http://www.scopus.com/inward/record.url?scp=85050377683&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050377683&partnerID=8YFLogxK

U2 - 10.1016/bs.mcb.2018.06.005

DO - 10.1016/bs.mcb.2018.06.005

M3 - Chapter

SN - 9780128142820

T3 - Methods in Cell Biology

SP - 41

EP - 57

BT - Methods in Cell Biology

A2 - Piel, Matthieu

A2 - Fletcher, Daniel

A2 - Doh, Junsang

PB - Academic Press Inc.

ER -

Bos J, Austin RH. A bacterial antibiotic resistance accelerator and applications. In Piel M, Fletcher D, Doh J, editors, Methods in Cell Biology. Academic Press Inc. 2018. p. 41-57. (Methods in Cell Biology). https://doi.org/10.1016/bs.mcb.2018.06.005