A 4.3-Mb YAC contig in human Xp11.2:Long-range restriction mapping and identification of CpG islands

Yong Wei, Ti Cai, Xiao Hui Wu, Yi Bin Kang, Jian Hua Chai

Research output: Contribution to journalArticle

Abstract

The Xp11.2 region of the human X chromosome contains genes involved in a number of inherited diseases, with at least one locus that escapes X chromosome inactivation, as well as abnormal methylation polymorphism. We isolated a series of yeast artificial chromosome (YAC) clones by hybridization screening with DNA probes localized within this region and assembled them into a 4.3Mb contig spanning from Xp11.2l to Xp11.23 by a combination of Alu-PCR fingerprinting, STS-PCR, and DNA probe cross hybridization. On the basis of these overlapping YAC clones we have constructed the long-range restriction map of this interval and placed exactly some DNA markers. Pour CpG-dense regions between ARAF1 and OATL2 were identified based on the long-range restriction mapping, which indicated the attribution of genes within this interval. It should assist in the future nucleic acid sequence analysis and novel gene identification in this region.

Original languageEnglish (US)
Pages (from-to)333-334
Number of pages2
JournalActa Biochimica et Biophysica Sinica
Volume28
Issue number4
StatePublished - Dec 1 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Keywords

  • CpG island
  • Long range physical map
  • X chromosome
  • YAC contig

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