A 3D culture model to study how fluid pressure and flow affect the behavior of aggregates of epithelial cells

Alexandra S. Piotrowski-Daspit, Allison K. Simi, Mei Fong Pang, Joe Tien, Celeste M. Nelson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cells are surrounded by mechanical stimuli in their microenvironment. It is important to determine how cells respond to the mechanical information that surrounds them in order to understand both development and disease progression, as well as to be able to predict cell behavior in response to physical stimuli. Here we describe a protocol to determine the effects of interstitial fluid flow on the migratory behavior of an aggregate of epithelial cells in a three-dimensional (3D) culture model. This protocol includes detailed methods for the fabrication of a 3D cell culture chamber with hydrostatic pressure control, the culture of epithelial cells as an aggregate in a collagen gel, and the analysis of collective cell behavior in response to pressure-driven flow.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages245-257
Number of pages13
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1501
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • 3D culture
  • Fluid flow
  • Fluid pressure
  • Mechanical stress
  • Micropatterning

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    Piotrowski-Daspit, A. S., Simi, A. K., Pang, M. F., Tien, J., & Nelson, C. M. (2017). A 3D culture model to study how fluid pressure and flow affect the behavior of aggregates of epithelial cells. In Methods in Molecular Biology (pp. 245-257). (Methods in Molecular Biology; Vol. 1501). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6475-8_12