2 Min-incubation and 6 pM-sensitivity microfluidic fluorescence immunoassay - A novel method for further significant enhancement using local electrical field on nanoplasmonic signal amplification surface

Ruoming Peng, Liangcheng Zhou, Qi Zhang, Wei Ding, Stephen Y. Chou

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Previously, we have demonstrated (a) a new nanoplasmonic surface (D2PA) [1] that enhances fluorescence assay (LoD) by 106 fold (to 300 aM) for 96-well-microtiter and 100 μL sample volume [2]; and (b) an microfluidic devices with D2PA inside microchannel that has a LoD enhancement of 104 fold (to 136 pM) for 120 nL sample volume and an incubation time reduction by 6 fold (to 20 mins)[3, 4]. Here we report a new method [5] that offers further significant enhancements over the previous enhancements, achieving additional 23-fold sensitivity increase (to 6 pM, 120 nL sample volume) and addtional 10 fold incubation time reduction (to 2 min), by creating local electrical field and gradient on D2PA.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1832-1834
Number of pages3
ISBN (Electronic)9780979806476
StatePublished - 2014
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Keywords

  • Acceleration
  • D2PA
  • Electric field
  • Enhanced sensitivity
  • Immunoassay

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  • Cite this

    Peng, R., Zhou, L., Zhang, Q., Ding, W., & Chou, S. Y. (2014). 2 Min-incubation and 6 pM-sensitivity microfluidic fluorescence immunoassay - A novel method for further significant enhancement using local electrical field on nanoplasmonic signal amplification surface. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1832-1834). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.