Abstract
Emerging evidence suggests functional regulation of the Hippo pathway by the actin cytoskeleton, although the detailed molecular mechanism remains incomplete. In a genetic screen, we identified a requirement for β-Spectrin in the posterior follicle cells for the oocyte repolarization process during Drosophila mid-oogenesis. β-spectrin mutations lead to loss of Hippo signaling activity in the follicle cells. A similar reduction of Hippo signaling activity was observed after β-Spectrin knockdown in mammalian cells. We further demonstrated that β-spectrin mutations disrupt the basal actin network in follicle cells. The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the β-spectrin mutations and the loss of the Hippo signaling activity phenotype.
Original language | English (US) |
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Pages (from-to) | 6397-6407 |
Number of pages | 11 |
Journal | Journal of Biological Chemistry |
Volume | 290 |
Issue number | 10 |
DOIs | |
State | Published - Mar 6 2015 |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Biochemistry
- Cell Biology