TY - JOUR
T1 - α integrin cytoplasmic tails have tissue-specific roles during C. elegans development
AU - Meighan, Christopher M.
AU - Schwarzbauer, Jean E.
N1 - Publisher Copyright:
© 2014 UBC Press.
PY - 2014
Y1 - 2014
N2 - Integrin signaling impacts many developmental processes. The complexity of these signals increases when multiple, unique integrin heterodimers are expressed during a single developmental event. Since integrin heterodimers have different signaling capabilities, the signals originating at each integrin type must be separated in the cell. C. elegans have two integrin heterodimers, α INA-1/β PAT-3 and α PAT-2/β PAT-3, which are expressed individually or simultaneously, based on tissue type. We used chimeric α integrins to assess the role of α integrin cytoplasmic tails during development. Chimeric integrin ina-1 with the pat-2 cytoplasmic tail rescued lethality and maintained neuron fasciculation in an ina-1 mutant. Interestingly, the pat-2 tail was unable to completely restore distal tip cell migration and vulva morphogenesis. Chimeric integrin pat-2 with the ina-1 cytoplasmic tail had a limited ability to rescue a lethal mutation in pat-2, with survivors showing aberrant muscle organization, yet normal distal tip cell migration. In a wild type background, α integrin pat-2 with the ina-1 cytoplasmic tail had a dominant negative effect which induced muscle disorganization, cell migration defects and lethality. These results show the a integrin cytoplasmic tails impact unique cellular behaviors that vary by tissue type during development.
AB - Integrin signaling impacts many developmental processes. The complexity of these signals increases when multiple, unique integrin heterodimers are expressed during a single developmental event. Since integrin heterodimers have different signaling capabilities, the signals originating at each integrin type must be separated in the cell. C. elegans have two integrin heterodimers, α INA-1/β PAT-3 and α PAT-2/β PAT-3, which are expressed individually or simultaneously, based on tissue type. We used chimeric α integrins to assess the role of α integrin cytoplasmic tails during development. Chimeric integrin ina-1 with the pat-2 cytoplasmic tail rescued lethality and maintained neuron fasciculation in an ina-1 mutant. Interestingly, the pat-2 tail was unable to completely restore distal tip cell migration and vulva morphogenesis. Chimeric integrin pat-2 with the ina-1 cytoplasmic tail had a limited ability to rescue a lethal mutation in pat-2, with survivors showing aberrant muscle organization, yet normal distal tip cell migration. In a wild type background, α integrin pat-2 with the ina-1 cytoplasmic tail had a dominant negative effect which induced muscle disorganization, cell migration defects and lethality. These results show the a integrin cytoplasmic tails impact unique cellular behaviors that vary by tissue type during development.
KW - Distal tip cell
KW - Integrin
KW - Morphogenesis
KW - Muscle
UR - http://www.scopus.com/inward/record.url?scp=84908112074&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908112074&partnerID=8YFLogxK
U2 - 10.1387/ijdb.130327cm
DO - 10.1387/ijdb.130327cm
M3 - Article
C2 - 25354452
AN - SCOPUS:84908112074
SN - 0214-6282
VL - 58
SP - 325
EP - 333
JO - International Journal of Developmental Biology
JF - International Journal of Developmental Biology
IS - 5
ER -