α-Fetoprotein and albumin genes are in tandem in the mouse genome

R. S. Ingram, R. W. Scott, S. M. Tilghman

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

The murine α-fetoprotein (AFP) and serum albumin genes most probably arose in evolution as the consequence of a duplication of a common ancestral gene. They have both been previously mapped to chromosome 5 in the mouse. We now have evidence that these genes are closely linked. By using a unique copy DNA probe derived from previously cloned AFP 5' flanking DNA, a recombinant DNA phage has been isolated, from a bacteriophage DNA library, that contains sequences flanking the 5' end of the AFP gene and the 3' end of the albumin gene. Restriction endonuclease mapping and DNA sequence determination of the recombinant phage and comparison to total genomic DNA confirmed that the genes are in tandem, 13.5 kilobase pairs apart, with the albumin gene to the 5' side of the AFP gene. Thus, they are transcribed from the same strand of DNA.

Original languageEnglish (US)
Pages (from-to)4694-4698
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume78
Issue number8 I
DOIs
StatePublished - 1981

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'α-Fetoprotein and albumin genes are in tandem in the mouse genome'. Together they form a unique fingerprint.

Cite this